Preparation of homogeneous solid samples for reproducible and quantitative MALDI

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Preparation of homogeneous solid samples for reproducible and quantitative MALDI
S H Ahn; K M Park; Jeong Hee Moon; S H Lee; M S Kim
Bibliographic Citation
Bulletin of Korean Chemical Society, vol. 37, pp. 458-462
Publication Year
Preparing samples with good homogeneity is one of the requirements for generating reproducible matrixassisted laser desorption ionization (MALDI) spectra and for using them in analyte quantification. Thus far, we have not been successful in producing homogeneous solid samples from popular matrixes apart from α-cyano-4-hydroxycinnamic acid (CHCA). In this work, we demonstrate the production of an outwardly homogeneous solid sample by loading a methanol solution of a matrix into a shallow reservoir on a coated MALDI sample plate and then vacuum-drying it. Out of ten popular matrixes tested, seven yielded homogeneous samples. These were 9-aminoacridine, 6-aza-2-thiothymine, CHCA, 2,5-dihydroxybenzoic acid, ferulic acid, sinapinic acid, and 2,4,6-trihydroxyacetophenone. For MALDI with these matrixes, linear calibration curves covering wide dynamic ranges were acquired. Performances of these matrixes in various aspects of analyte quantification have been investigated.
MALDI quantificationMALDI temperatureMatrix suppressionMatrix-assisted laser desorption ionizationReproducible MALDI
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Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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