Structural features of Cas2 from Thermococcus onnurineus in CRISPR-cas system type IV

Cited 6 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorTae-Yang Jung-
dc.contributor.authorKwang Hyun Park-
dc.contributor.authorYan An-
dc.contributor.authorA Schulga-
dc.contributor.authorS Deyev-
dc.contributor.authorJ H Jung-
dc.contributor.authorEui-jeon Woo-
dc.date.accessioned2017-04-19T10:30:22Z-
dc.date.available2017-04-19T10:30:22Z-
dc.date.issued2016-
dc.identifier.issn09618368-
dc.identifier.uri10.1002/pro.2981ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/13559-
dc.description.abstractCRISPR-Cas is RNA-based prokaryotic immune systems that defend against exogenous genetic elements such as plasmids and viruses. Cas1 and Cas2 are highly conserved components that play an essential part in the adaptation stage of all CRISPR-Cas systems. Characterization of CRISPR-Cas genes in Thermococcus onnurineus reveals the association of the Cas2 gene with the putative type IV system that lacks Cas1 or its homologous genes. Here, we present a crystal structure of T. onnurineus Cas2 (Ton_Cas2) that exhibits a deep and wide cleft at an interface lined with positive residues (Arg16, Lys18, Lys19, Arg22, and Arg23). The obvious DNA recognizing loops in Cas2 from E. coli (Eco_Cas2) are absent in Ton_Cas2 and have significantly different shapes and electrostatic potential distributions around the putative nucleotide binding region. Furthermore, Ton_Cas2 lacks the hairpin motif at the C-terminus that is responsible for Cas1 binding in Eco_Cas2. These structural features could be a unique signature and indicate an altered functional mechanism in the adaptation stage of Cas2 in type IV CRISPR-Cas systems-
dc.publisherWiley-
dc.titleStructural features of Cas2 from Thermococcus onnurineus in CRISPR-cas system type IV-
dc.title.alternativeStructural features of Cas2 from Thermococcus onnurineus in CRISPR-cas system type IV-
dc.typeArticle-
dc.citation.titleProtein Science-
dc.citation.number0-
dc.citation.endPage1897-
dc.citation.startPage1890-
dc.citation.volume25-
dc.contributor.affiliatedAuthorEui-jeon Woo-
dc.contributor.alternativeName정태양-
dc.contributor.alternativeName박광현-
dc.contributor.alternativeName안연-
dc.contributor.alternativeNameSchulga-
dc.contributor.alternativeNameDeyev-
dc.contributor.alternativeName정종현-
dc.contributor.alternativeName우의전-
dc.identifier.bibliographicCitationProtein Science, vol. 25, pp. 1890-1897-
dc.identifier.doi10.1002/pro.2981-
dc.subject.keywordCas2-
dc.subject.keywordCRISPR-Cas system-
dc.subject.keywordprotein structure-
dc.subject.keywordThermococcus onnurineus-
dc.subject.keywordtype IV system-
dc.subject.localCas2-
dc.subject.localCRISPR-Cas system-
dc.subject.localprotein structure-
dc.subject.localProtein structure-
dc.subject.localThermococcus onnurineus-
dc.subject.localtype IV system-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.