Melamine and cyanuric acid co-exposure causes renal dysfunction and structural damage via MAPKs and mitochondrial signaling

Abstract

Both melamine and cyanuric acid have low toxicity, but combined exposure to melamine and cyanuric acid (M+CA) was reported to cause unexpected toxicological synergy on kidney damage. In this study, we investigated the role of oxidative stress and apoptotic changes in the nephrotoxicity caused by M+CA in rats. M+CA treatment caused an increase in the kidney weight, serum blood urea nitrogen or creatinine levels in a dose-dependent manner. With severe morphological and histological changes in kidneys, M+CA markedly elevated lipid peroxidation and suppressed antioxidant enzyme activities. M+CA treatment induced apoptotic changes in renal tubular cells. Additionally, exposure to M+CA increased phosphorylation of mitogen-activated protein kinases (MAPKs) coincided with increased Bax level and decreased Bcl2 level, resulting in caspase-3 activation. In summary, oxidative stress and simultaneous MAPKs and mitochondrial/caspase activation may be related to renal tubular cell apoptosis caused by M+CA