Paracoccus aestuariivivens sp. nov., isolated from a tidal flat

Abstract

A Gram-stain-negative, non-spore-forming, non-motile and coccoid, ovoid or short-rod-shaped bacterial strain, designated GHD-30T, was isolated from a tidal flat on the Yellow Sea in South Korea, and subjected to a polyphasic taxonomic study. Strain GHD-30T grew optimally at 30 °C, at pH 6.5-7.5 and in the presence of 2.0% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain GHD-30Trepresented a member of the genus Paracoccus. Strain GHD-30T exhibited 16S rRNA gene sequence similarity values of 97.10-97.33% to the type strains of Paracoccus lutimaris, Paracoccus limosus and Paracoccus halophilus and of 94.35-96.97% to the type strains of the other species of the genus Paracoccus. Strain GHD-30T contained Q-10 as the predominant ubiquinone and C18:1ω7c as the major fatty acid. The major polar lipids detected in strain GHD-30T were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified aminolipid. The DNA G+C content of strain GHD-30T was 62.0 mol% and its mean DNA-DNA relatedness values with the type strains of P. lutimaris, P. limosus, and P. halophilus were 11-19 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GHD-30T is separated from recognized species of the genus Paracoccus. On the basis of the data presented, strain GHD-30T is considered to represent a novel species of the genus Paracoccus, for which the name Paracoccus aestuariivivens sp. nov. is proposed. The type strain is GHD-30T (=KCTC 52214T =NBRC 111993T).