Effect of high hydrostatic pressure treatment on isoquercetin production from rutin by commercial α-L-rhamnosidase = 엘 람노시다아제를 이용한 루틴으로부터 이소쿼세틴 생산성에 정수압이 미치는 영향

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Title
Effect of high hydrostatic pressure treatment on isoquercetin production from rutin by commercial α-L-rhamnosidase = 엘 람노시다아제를 이용한 루틴으로부터 이소쿼세틴 생산성에 정수압이 미치는 영향
Author(s)
D Y Kim; Soo Jin Yeom; C S Park; Y S Kim
Bibliographic Citation
Biotechnology Letters, vol. 38, pp. 1775-1780
Publication Year
2016
Abstract
Objectives: To optimize conversion of rutin to isoquercetin by commercial α-l-rhamnosidase using high hydrostatic pressure (HHP). Results: The de-rhamnosylation activity of α-l-rhamnosidase for isoquercetin production was maximal at pH 6.0 and 50?°C using HHP (150 MPa). The enzyme showed high specificity for rutin. The specific activity for rutin at HHP was 1.5-fold higher than that at atmospheric pressure. The enzyme completely hydrolysed 20 mM rutin in tartary buckwheat extract after 2 h at HHP, with a productivity of 10 mM h?1. The productivity and conversion were 2.2- and 1.5-fold higher at HHP than at atmospheric pressure, respectively. Conclusions: This is the first report concerning the enzymatic hydrolysis of isoquercetin in tartary buckwheat at HHP.
Keyword
Hydrostatic pressureIsoquercetinRutinTartary buckwheatα-l-rhamnosidase
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-016-2157-5
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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