Inhibitory effects of IL-6-induced STAT3 activation of bio-activecompounds derived from Salvia plebeia R.Br = 배암차즈기로부터 분리된 화합물들의 IL-6 유도 STAT3 활성화 억제효과

Abstract

Salvia plebeia R.Br. (SPRB) is a natural resource that exhibits various biological activities. However,its effects on inflammatory diseases are not yet well characterized. This study aimed to evaluate theinhibitory effects of a compound derived from SPRB on interleukin-6 (IL-6)/signal transducer and acti-vator of transcription 3 (STAT3) activation. The chemical structure of active compounds isolated fromthe EtOAc-soluble fraction of SPRB were elucidated by comparison with spectroscopic nuclear magneticresonance (NMR) and electrospray ionization mass spectrometry (ESI？MS) data in the literature, whichresulted in the identification of 22 known compounds. All of the isolated compounds were evaluated forpotentially higher inhibitory effects on IL-6-induced STAT3 activation in Hep3B cells using a luciferasereporter assay. Furthermore, the protein levels of p-STAT3, p-ERK, and p-JAK2 in IL-6 induced U266 cellswere regulated in the presence of cirsimaritin from SPRB by western blot assay. Based on these findings,we suggest that SPRB has the potential to inhibit IL-6-induced STAT3 activation and should be consideredin functional food and pharmaceutical applications.