Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

Cited 43 time in scopus
Metadata Downloads
Title
Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts
Author(s)
J H Kim; C Wang; H J Jang; M S Cha; J E Park; S Y Jo; Eui Sung Choi; S W Kim
Bibliographic Citation
Microbial Cell Factories, vol. 15, pp. 214-214
Publication Year
2016
Abstract
Background: Isoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives. Results: In this study, Escherichia coli MG1655 harboring Populus trichocarpa isoprene synthase (PtispS) and the exogenous mevalonate (MVA) pathway produced 80mg/L isoprene. Codon optimization and optimal expression of the ispS gene via adjustment of the RBS strength and inducer concentration increased isoprene production to 199 and 337mg/L, respectively. To augment expression of MVA pathway genes, the MVA pathway was cloned on a high-copy plasmid (pBR322 origin) with a strong promoter (Ptrc), which resulted in an additional increase in isoprene production up to 956mg/L. To reduce the formation of byproducts derived from acetyl-CoA (an initial substrate of the MVA pathway), nine relevant genes were deleted to generate the E. coli AceCo strain (E. coli MG1655 ?ackA-pta, poxB, ldhA, dld, adhE, pps, and atoDA). The AceCo strain harboring the ispS gene and MVA pathway showed enhanced isoprene production of 1832mg/L in flask culture with reduced accumulation of byproducts. Conclusions: We achieved a 23-fold increase in isoprene production by codon optimization of PtispS, augmentation of the MVA pathway, and deletion of genes involved in byproduct formation
Keyword
BioisopreneCarbon utilizationEscherichia coliIsoprene synthaseMevalonate pathway
ISSN
1475-2859
Publisher
Springer-BMC
DOI
http://dx.doi.org/10.1186/s12934-016-0612-6
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.