Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor

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Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor
L Jin; So Ra Ko; Yingshun Cui; Chang Soo Lee; Hee-Mock OhChi-Yong AhnHyung Gwan Lee
Bibliographic Citation
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, vol. 110, no. 1, pp. 125-132
Publication Year
A polyphasic taxonomic study was carried out on strain EBR-8-1T isolated from a biofilm reactor in Korea. The cells of the strain were Gram-stain negative, non-spore-forming, non-motile, and short rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain with Betaproteobacteria, which showed high pairwise sequence similarities with Pusillimonas noertemannii BN9T (99.1?%), Pusillimonas soli MJ07T (97.3?%), Pusillimonas ginsengisoli DCY25T (97.2?%), and Pusillimonas harenae B201T (96.8?%). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage within the genus Pusillimonas. The major fatty acids were identified as C16:0, C17:0 cyclo and C19:0 cyclo ω8c. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The respiratory quinone was identified as Q-8 and the genomic DNA G+C content was determined to be 63.3?mol%. On the basis of polyphasic evidence, it is proposed that strain EBR-8-1T should be placed in a new species, Pusillimonas caeni sp. nov. The type stain is EBR-8-1T (=KCTC 42353T?=?JCM 30463T).
Biofilm reactorEBR-8-1TPusillimonasPusillimonas caeni
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Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
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