One-step purification of melittin derived from Apis mellifera bee venom

Cited 4 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorA C L Teoh-
dc.contributor.authorKyoung Hwa Ryu-
dc.contributor.authorEun Gyo Lee-
dc.date.accessioned2017-08-29-
dc.date.available2017-08-29-
dc.date.issued2017-
dc.identifier.issn10177825-
dc.identifier.uri10.4014/jmb.1608.08042ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17038-
dc.description.abstractThe concern over the use of melittin in honey bee venom due to its adverse reaction caused by allergens such as phospholipase A2 (PLA) and hyaluronidase (HYA) has been an obstacle towards its usage. We developed a novel single-step method for melittin purification and the2 removal of PLA and HYA. This study explores the influence of pH, buffer compositions, salt concentration, and types of cation-exchange chromatography resins on the recovery of2 melittin and the removal of both HYA and PLA. Melittin was readily purified with a strong cation-exchange resin at pH 6.0 with sodium phosphate buffer. It resulted in a recovery yield2 of melittin up to 93% (5.87 mg from a total of 6.32 mg of initial melittin in crude bee venom), which is higher than any previously reported studies on melittin purification. PLA (99%) and HYA (96%) were also successfully removed. Our study generates a single-step purification2 method for melittin with a high removal rate of PLA and HYA, enabling melittin to be fully utilized for its therapeutic purposes.-
dc.publisherSouth Korea-
dc.titleOne-step purification of melittin derived from Apis mellifera bee venom-
dc.title.alternativeOne-step purification of melittin derived from Apis mellifera bee venom-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number1-
dc.citation.endPage91-
dc.citation.startPage84-
dc.citation.volume27-
dc.contributor.affiliatedAuthorKyoung Hwa Ryu-
dc.contributor.affiliatedAuthorEun Gyo Lee-
dc.contributor.alternativeNameTeoh-
dc.contributor.alternativeName류경화-
dc.contributor.alternativeName이은교-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 27, no. 1, pp. 84-91-
dc.identifier.doi10.4014/jmb.1608.08042-
dc.subject.keywordCation-exchange chromatography-
dc.subject.keywordHyaluronidase-
dc.subject.keywordMelittin-
dc.subject.keywordPhospholipase A2-
dc.subject.keywordPurification-
dc.subject.localCation-exchange chromatography-
dc.subject.localCation exchange chromatography-
dc.subject.localHyaluronidase-
dc.subject.localMelittin-
dc.subject.localPhospholipase A2-
dc.subject.localPurification-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.