Abyssicoccus albus gen. nov., sp. nov., a novel member of the family Staphylococcaceae isolated from marine sediment of the Indian Ocean

Abstract

A Gram-stain positive, aerobic, non-motile, asporogenous, coccoid shaped bacterium, designated YIM M12140T, was isolated from a marine sediment sample collected from the Indian Ocean. Phylogenetic analysis showed that strain YIM M12140Tforms a separate clade within the family Staphylococcaceae. Strain YIM M12140Tshares high 16S rRNA gene sequence similarity with Macrococcusbrunensis DSM 19358T(92.9%). The isolate was found to grow at 0-10% (w/v) NaCl (optimum, 2-3%), pH 6.0-10.0 (optimum, pH 8.0) and temperature 5-40°C (optimum, 28°C). The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid and two unidentified polar lipids. The major cellular fatty acids of the strain were identified as anteiso-C15:0, -C17:0, iso-C16:0, anteiso-C19:0and C20:0. The respiratory menaquinones were found to be MK-6 (94%) and MK-7 (6%). The cell wall amino acids were found to contain Lys, Ala, Glu, Gly, Asp, Ser and Thr. Whole cell sugars were identified as mannose, ribose, rhamnose, glucose, galactose and xylose. The G+C content of the genomic DNA of strain YIM M12140Twas determined to be 42.4 mol%. Based on phenotypic, chemotaxonomic data and phylogenetic analysis, it is proposed that strain YIM M12140Trepresents a novel species of a new genus in the family Staphylococcaceae, for which the name Abyssicoccus albus gen. nov., sp. nov. is proposed. The type strain is YIM M12140T(= DSM 29158T=CCTCC AB 2014213T).