Down-regulation of GIGANTEA-like genes increases plant growth and salt stress tolerance in poplar

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Title
Down-regulation of GIGANTEA-like genes increases plant growth and salt stress tolerance in poplar
Author(s)
Q Ke; Ho Soo Kim; Z Wang; Chang Yoon Ji; Jae Cheol Jeong; Haeng Soon Lee; Y I Choi; B Xu; X Deng; D J Yun; Sang Soo Kwak
Bibliographic Citation
Plant Biotechnology Journal, vol. 15, no. 3, pp. 331-343
Publication Year
2017
Abstract
The flowering time regulator GIGANTEA (GI) connects networks involved in developmental stage transitions and environmental stress responses in Arabidopsis. However, little is known about the role of GI in growth, development and responses to environmental challenges in the perennial plant poplar. Here, we identified and functionally characterized three GI-like genes (PagGIa, PagGIb and PagGIc) from poplar (Populus alba?×?Populus glandulosa). PagGIs are predominantly nuclear localized and their transcripts are rhythmically expressed, with a peak around zeitgeber time 12 under long-day conditions. Overexpressing PagGIs in wild-type (WT) Arabidopsis induced early flowering and salt sensitivity, while overexpressing PagGIs in the gi-2 mutant completely or partially rescued its delayed flowering and enhanced salt tolerance phenotypes. Furthermore, the PagGIs-PagSOS2 complexes inhibited PagSOS2-regulated phosphorylation of PagSOS1 in the absence of stress, whereas these inhibitions were eliminated due to the degradation of PagGIs under salt stress. Down-regulation of PagGIs by RNA interference led to vigorous growth, higher biomass and enhanced salt stress tolerance in transgenic poplar plants. Taken together, these results indicate that several functions of Arabidopsis GI are conserved in its poplar orthologues, and they lay the foundation for developing new approaches to producing salt-tolerant trees for sustainable development on marginal lands worldwide.
Keyword
ArabidopsisPagGIpoplarRNAisalt tolerancetransgenic plants
ISSN
1467-7644
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/pbi.12628
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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