Rapid and reliable species identification of Carposina sasakii from its morphological homologues, by MALDI-TOF mass spectrometry

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Title
Rapid and reliable species identification of Carposina sasakii from its morphological homologues, by MALDI-TOF mass spectrometry
Author(s)
Jun Hyoung Jeon; Hyun Woo OhChan Seok Yun; B K Byun; J J Park; H S Park; Doo-Sang Park
Bibliographic Citation
Journal of Asia-Pacific Entomology, vol. 20, no. 2, pp. 411-415
Publication Year
2017
Abstract
Rapid and accurate identification of pest species found on quarantine inspection is of great importance to prevent an economic loss of agricultural commodities. In this study, we tested MALDI-TOF mass spectrometry to discriminate the larvae of three morphologically similar pest species, Carposina sasakii, Grapholita molesta and Grapholita dimorpha, which usually found in Korean fruit exported to Asia and western countries. Unique mass spectra profile by MALDI-TOF MS covering a wide molecular mass range of 2000?20,000 Da were produced from twelve reference samples for three species. The Principal Component Analysis and the heat map profile showed similar pattern within same species regardless of body part of a larvae, however showed clear discrimination between different species. This identification method can discriminate species within 15 min with a relatively small sample sized 2 mm3. It also can provide a simple well documented protocol and can be widely applied to identify pests that essentially needed an urgent identification such as quarantine inspection or health related pest monitoring to reduce an economic loss or to control the spread of the disease.
Keyword
C. sasakiiG. dimorphaG. molestaMALDI-TOF MSQuarantine pestSpecies identification
ISSN
I000-0143
Publisher
South Korea
DOI
http://dx.doi.org/10.1016/j.aspen.2017.02.013
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Facility Management Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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