Physiological mechanism of enhanced salt stress tolerance in sweet potato (Ipomoes batats L.) with overexpression of IbOr gene

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Title
Physiological mechanism of enhanced salt stress tolerance in sweet potato (Ipomoes batats L.) with overexpression of IbOr gene
Author(s)
L Zhang; H Li; Z Sun; S Wang; Sang Soo Kwak; X Deng
Bibliographic Citation
Chinese Journal of Applied and Environmental Biology, vol. 23, no. 1, pp. 54-59
Publication Year
2017
Abstract
Sweet potato is one of the world's most important food, feed, industrial materials and bioenergy crops. Comprehensive understanding of the physiological mechanism of whether and how transgenic sweet potato overexpressing IbOr genes enhance the tolerance to salt stress and developing a strong salt tolerant sweet potato has important theoretical and practical significance for effective utilization of saline land and alleviation of the energy crisis. Transgenic (TS) and non-transgenic (NS) sweet potato plants were treated with 150 mmol/L NaCl salt stress. The changes of photosynthetic parameters and the activities of antioxidant enzyme were studied at different times after salinity treatment. With salt stress time extended, chlorophyll and carotenoids content, photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), transpiration rate (Tr) all decreased evidently in both TS and NS sweet potato groups, though only slightly in TS lines. After three days of salt stress, the contents of O2-·and MDA in TS were 61.23 μg/g FW and 22.51 μmol/g FW, respectively. While the contents of O2-· and MDA in NS reached 80.56 μg/g FW and 31.92 μmol/g FW. Compared with the NS, TS plants had lower level of O2-· and malondialdehyde (MDA) contents. After salinity treatment, the activities of SOD, POD and CAT all firstlyincreased, and then decreased slightly, with significantly higher enzyme activities in TS lines than in NT plants. After nine days of salt stress, the Na+ contents in TS and NS reached 25.44 mg/g DW and 35.08 mg/g DW, being 11.47 and 14.83 times of that before treatment, respectively. The Na+ content increased evidently in both but significantly higher in NT plants after salt stress. The results indicated that TS plants had lower reactive oxygen species (ROS) level and lipid membrane damage than TS plants. The higher contents of chlorophyll and carotenoids in TS plants may lead to a higher photosynthetic ability than NT plants. Enhancement of salt tolerance in transgenic sweet potato may be achieved mostly by improving antioxidant ability of sweet potato.
Keyword
Antioxidant enzymeCarotenoidsNet photosynthetic rateSalt stressTransgenic sweet potatoTranspiration rate
ISSN
1006-687X
Publisher
South Korea
DOI
http://dx.doi.org/10.3724/SP.J.1145.2016.05005
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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