Dual effect of fetal bovine serum on early development depends on stage-specific reactive oxygen species demands in pigs

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dc.contributor.authorSeong-Eun Mun-
dc.contributor.authorBo Woong Sim-
dc.contributor.authorSeung-Bin Yoon-
dc.contributor.authorPil Soo Jeong-
dc.contributor.authorHae Jun Yang-
dc.contributor.authorSeon A Choi-
dc.contributor.authorYoung-Ho Park-
dc.contributor.authorYoung-Hyun Kim-
dc.contributor.authorPhilyong Kang-
dc.contributor.authorKang Jin Jeong-
dc.contributor.authorYoungjeon Lee-
dc.contributor.authorYeung Bae Jin-
dc.contributor.authorBong-Seok Song-
dc.contributor.authorJi-Su Kim-
dc.contributor.authorJae Won Huh-
dc.contributor.authorSang-Rae Lee-
dc.contributor.authorY K Choo-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorKyu Tae Chang-
dc.date.accessioned2017-08-29-
dc.date.available2017-08-29-
dc.date.issued2017-
dc.identifier.issn1932-6203-
dc.identifier.uri10.1371/journal.pone.0175427ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17103-
dc.description.abstractDespite the application of numerous supplements to improve in vitro culture (IVC) conditions of mammalian cells, studies regarding the effect of fetal bovine serum (FBS) on mammalian early embryogenesis, particularly in relation to redox homeostasis, are lacking. Herein, we demonstrated that early development of in vitro-produced (IVP) porcine embryos highly depends on the combination of FBS supplementation timing and embryonic reactive oxygen species (ROS) requirements. Interestingly, FBS significantly reduced intracellular ROS levels in parthenogenetically activated (PA) embryos regardless of the developmental stage. However, the beneficial effect of FBS on early embryogenesis was found only during the late phase (IVC 4-6 days) treatment group. In particular, developmental competence parameters, such as blastocyst formation rate, cellular survival, total cell number and trophectoderm proportion, were markedly increased by FBS supplementation during the late IVC phase. In addition, treatment with FBS elevated antioxidant transcript levels during the late IVC phase. In contrast, supplementation with FBS during the entire period (1-6 days) or during the early IVC phase (1-2 days) greatly impaired the developmental parameters. Consistent with the results from PA embryos, the developmental competence of in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) embryos were markedly improved by treatment with FBS during the late IVC phase. Moreover, the embryonic stage-specific effects of FBS were reversed by the addition of an oxidant and were mimicked by treatment with an antioxidant. These findings may increase our understanding of redox-dependent early embryogenesis and contribute to the large-scale production of high-quality IVP embryos.-
dc.publisherPublic Library of Science-
dc.titleDual effect of fetal bovine serum on early development depends on stage-specific reactive oxygen species demands in pigs-
dc.title.alternativeDual effect of fetal bovine serum on early development depends on stage-specific reactive oxygen species demands in pigs-
dc.typeArticle-
dc.citation.titlePLoS One-
dc.citation.number4-
dc.citation.endPagee0175427-
dc.citation.startPagee0175427-
dc.citation.volume12-
dc.contributor.affiliatedAuthorSeong-Eun Mun-
dc.contributor.affiliatedAuthorBo Woong Sim-
dc.contributor.affiliatedAuthorSeung-Bin Yoon-
dc.contributor.affiliatedAuthorPil Soo Jeong-
dc.contributor.affiliatedAuthorHae Jun Yang-
dc.contributor.affiliatedAuthorSeon A Choi-
dc.contributor.affiliatedAuthorYoung-Ho Park-
dc.contributor.affiliatedAuthorYoung-Hyun Kim-
dc.contributor.affiliatedAuthorPhilyong Kang-
dc.contributor.affiliatedAuthorKang Jin Jeong-
dc.contributor.affiliatedAuthorYoungjeon Lee-
dc.contributor.affiliatedAuthorYeung Bae Jin-
dc.contributor.affiliatedAuthorBong-Seok Song-
dc.contributor.affiliatedAuthorJi-Su Kim-
dc.contributor.affiliatedAuthorJae Won Huh-
dc.contributor.affiliatedAuthorSang-Rae Lee-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorKyu Tae Chang-
dc.contributor.alternativeName문성은-
dc.contributor.alternativeName심보웅-
dc.contributor.alternativeName윤승빈-
dc.contributor.alternativeName정필수-
dc.contributor.alternativeName양해준-
dc.contributor.alternativeName최선아-
dc.contributor.alternativeName박영호-
dc.contributor.alternativeName김영현-
dc.contributor.alternativeName강필용-
dc.contributor.alternativeName정강진-
dc.contributor.alternativeName이영전-
dc.contributor.alternativeName진영배-
dc.contributor.alternativeName송봉석-
dc.contributor.alternativeName김지수-
dc.contributor.alternativeName허재원-
dc.contributor.alternativeName이상래-
dc.contributor.alternativeName추영국-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName장규태-
dc.identifier.bibliographicCitationPLoS One, vol. 12, no. 4, pp. e0175427-e0175427-
dc.identifier.doi10.1371/journal.pone.0175427-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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