Lactococcus lactis KR-050L inhibit IL-6/STAT3 activation = 락토바실러스 락티스 KR-050L의 IL-6/STAT3 저해활성

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Title
Lactococcus lactis KR-050L inhibit IL-6/STAT3 activation = 락토바실러스 락티스 KR-050L의 IL-6/STAT3 저해활성
Author(s)
Joo Tae Hwang; Hyun Jae Jang; Jin Han Kim; Chan Sun Park; Ye Sol Kim; C H Lim; Seung Woong LeeMun Chual Rho
Bibliographic Citation
Journal of Applied Microbiology, vol. 122, no. 5, pp. 1412-1422
Publication Year
2017
Abstract
Aims: The purpose of this study was to investigate IL-6/STAT3 inhibitory activity using lactic acid bacteria (LABs) isolated from Gajuknamu kimchi. Methods and Results: Six LABs were isolated from Gajuknamu kimchi and identified through 16S rRNA sequencing. Among them, the culture broth of Lactococcus lactis KR-050L inhibited IL-6-induced STAT3 luciferase activity. Fifteen compounds were isolated from the EtOAc extract of culture broth though column chromatography and preparative high-performance liquid chromatography, and they were identified as 2,5-diketopipperazine structures by spectroscopic analyses (MS, 1H- and 13C-NMR). They also showed inhibitory activities on IL-6-induced STAT3 activation, and showed the different in activity according to the presence of a phenylalanine residue, hydroxyl groups and isometric structure. Conclusions: The six new LABs isolated from Gajuknamu kimchi, and Lc.?lactis KR-050L was selected as candidate IL-6/STAT3 inhibitors. The activity levels of 15 2,5-DKPs isolated from Lc. lactis KR-050L were verified. Significance and Impact of the Study: This study constitutes the first attempt to isolate various LABs from Gajuknamu kimchi and to discover IL-6/STAT3 inhibitors in the EtOAc extract of Lc. lactis KR-050L culture broth. Moreover, our data provide useful biochemical information regarding the commercialization of Lc. lactis isolated from Gajuknamu kimchi as an approach to use functional foods for the treatment of various diseases via IL-6/STAT3 activation
Keyword
2,5-diketopipperazineGajuknamu kimchiIL-6Lactococcus lactis KR-050LSTAT3
ISSN
1364-5072
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/jam.13444
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
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