Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR = 실시간 유전자 증폭법을 활용한 사스, 메르스 및 관련 박쥐 코로나바이러스 동시검출 기법

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dc.contributor.authorJi Yeong Noh-
dc.contributor.authorSun-Woo Yoon-
dc.contributor.authorDoo-Jin Kim-
dc.contributor.authorMoo-Seung Lee-
dc.contributor.authorJi-Hyung Kim-
dc.contributor.authorW Na-
dc.contributor.authorD Song-
dc.contributor.authorDae Gwin Jeong-
dc.contributor.authorHye Kwon Kim-
dc.date.accessioned2017-08-29-
dc.date.available2017-08-29-
dc.date.issued2017-
dc.identifier.issn0304-8608-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17133-
dc.description.abstractSince severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50-100 copies/mL and 5-100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats. ⓒ 2017, Springer-Verlag Wien.-
dc.publisherSpringer-
dc.titleSimultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR = 실시간 유전자 증폭법을 활용한 사스, 메르스 및 관련 박쥐 코로나바이러스 동시검출 기법-
dc.title.alternativeSimultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR-
dc.typeArticle-
dc.citation.titleArchives of Virology-
dc.citation.number6-
dc.citation.endPage1623-
dc.citation.startPage1617-
dc.citation.volume162-
dc.contributor.affiliatedAuthorJi Yeong Noh-
dc.contributor.affiliatedAuthorSun-Woo Yoon-
dc.contributor.affiliatedAuthorDoo-Jin Kim-
dc.contributor.affiliatedAuthorMoo-Seung Lee-
dc.contributor.affiliatedAuthorJi-Hyung Kim-
dc.contributor.affiliatedAuthorDae Gwin Jeong-
dc.contributor.affiliatedAuthorHye Kwon Kim-
dc.contributor.alternativeName노지영-
dc.contributor.alternativeName윤선우-
dc.contributor.alternativeName김두진-
dc.contributor.alternativeName이무승-
dc.contributor.alternativeName김지형-
dc.contributor.alternativeName나운성-
dc.contributor.alternativeName송대섭-
dc.contributor.alternativeName정대균-
dc.contributor.alternativeName김혜권-
dc.identifier.bibliographicCitationArchives of Virology, vol. 162, no. 6, pp. 1617-1623-
dc.identifier.doi10.1007/s00705-017-3281-9-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
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