DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ji Yeong Noh | - |
dc.contributor.author | Sun-Woo Yoon | - |
dc.contributor.author | Doo-Jin Kim | - |
dc.contributor.author | Moo-Seung Lee | - |
dc.contributor.author | Ji-Hyung Kim | - |
dc.contributor.author | W Na | - |
dc.contributor.author | D Song | - |
dc.contributor.author | Dae Gwin Jeong | - |
dc.contributor.author | Hye Kwon Kim | - |
dc.date.accessioned | 2017-08-29 | - |
dc.date.available | 2017-08-29 | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0304-8608 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/17133 | - |
dc.description.abstract | Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50-100 copies/mL and 5-100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats. ⓒ 2017, Springer-Verlag Wien. | - |
dc.publisher | Springer | - |
dc.title | Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR = 실시간 유전자 증폭법을 활용한 사스, 메르스 및 관련 박쥐 코로나바이러스 동시검출 기법 | - |
dc.title.alternative | Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR | - |
dc.type | Article | - |
dc.citation.title | Archives of Virology | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 1623 | - |
dc.citation.startPage | 1617 | - |
dc.citation.volume | 162 | - |
dc.contributor.affiliatedAuthor | Ji Yeong Noh | - |
dc.contributor.affiliatedAuthor | Sun-Woo Yoon | - |
dc.contributor.affiliatedAuthor | Doo-Jin Kim | - |
dc.contributor.affiliatedAuthor | Moo-Seung Lee | - |
dc.contributor.affiliatedAuthor | Ji-Hyung Kim | - |
dc.contributor.affiliatedAuthor | Dae Gwin Jeong | - |
dc.contributor.affiliatedAuthor | Hye Kwon Kim | - |
dc.contributor.alternativeName | 노지영 | - |
dc.contributor.alternativeName | 윤선우 | - |
dc.contributor.alternativeName | 김두진 | - |
dc.contributor.alternativeName | 이무승 | - |
dc.contributor.alternativeName | 김지형 | - |
dc.contributor.alternativeName | 나운성 | - |
dc.contributor.alternativeName | 송대섭 | - |
dc.contributor.alternativeName | 정대균 | - |
dc.contributor.alternativeName | 김혜권 | - |
dc.identifier.bibliographicCitation | Archives of Virology, vol. 162, no. 6, pp. 1617-1623 | - |
dc.identifier.doi | 10.1007/s00705-017-3281-9 | - |
dc.description.journalClass | Y | - |
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