Naked eye detection of Salmonella typhimurium using scanometric antibody probe

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Naked eye detection of Salmonella typhimurium using scanometric antibody probe
So Yeon Yi; Seul Gee Hwang; Jeong Moon; G Eom; A Hwang; Jieun Shim; Eun Kyung LimJin Young Jeong; B Kim; Taejoon KangJuyeon Jung
Bibliographic Citation
Journal of Nanoscience and Nanotechnology, vol. 17, no. 7, pp. 4608-4612
Publication Year
Salmonella is one of the most common foodborne pathogens, and Salmonella outbreaks are mostly associated with the intake of contaminated food or drink. Therefore, the rapid and sensitive on-site detection of Salmonella is very important. We report a naked eye detection method for Salmonella typhimurium using scanometric antibody probe. The antibody-attached glass substrate was treated with Salmonella typhimurium and the scanometric antibody probe was applied. After Ag enhancement of the probe, Salmonella typhimurium could be detected with the naked eye. The scanometric antibody probe was prepared by simply mixing Au nanoparticles, gold binding peptide-protein G, and antibody against Salmonella typhimurium. This probe can act as a signal enhancer and thus allows for an extremely simple, rapid, and efficient analysis of Salmonella typhimurium by the naked eye. We detected Salmonella typhimurium at a low concentration of 103 CFU/ml and clearly distinguished this bacterium from other foodborne pathogens. Furthermore, we successfully detected Salmonella typhimurium in milk, suggesting that this method can be useful in real-life samples. Because the scanometric antibody probe can be expanded to various types of antibodies, this naked eye detection method could be employed for the detection of various types of pathogens.
AntibodyGold binding peptideNaked eyeProtein GSalmonella
Amer Scientific Publishers
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
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