Reduced gene expression at the branch point of chlorophyll and heme biosynthesis in Arctic Chlorella ArM0029B

Cited 2 time in scopus
Metadata Downloads
Title
Reduced gene expression at the branch point of chlorophyll and heme biosynthesis in Arctic Chlorella ArM0029B
Author(s)
V?Jayaraman; Kwon Hwangbo; Jong Min LimSung Ran Min; J W Ahn; D W Choi; Won Joong Jeong
Bibliographic Citation
Plant Biotechnology Reports, vol. 11, no. 1, pp. 9-15
Publication Year
2017
Abstract
The gene expression at the branch point of chlorophyll and heme synthesis in the model microalga, Chlamydomonas reinhardtii, is different from that of higher plants. Another green alga, Arctic Chlorella, was recently isolated from Arctic sea ice and may be a promising candidate for a biofuel. To understand the chlorophyll metabolic pathway and relevant nuclear gene expression in Chlorella sp., we characterized chlorophyll-deficient mutants of the Arctic Chlorella sp. ArM0029B. First, we characterized the chlorophyll and heme biosynthetic pathways based on genes identified by bioinformatics analysis of the genome of Arctic Chlorella sp. ArM0029B. Then, we isolated and analyzed nine chlorophyll-deficient mutants that showed reduced expression of the ChlM gene, which encodes Mg-protoporphyrin methyltransferase. Expression of 5-amino levulinic acid dehydratase (encoded by ALAD) and glutamyl-tRNA reductase (encoded by HemA) was reduced in all nine independent mutants compared to wild type. These results indicated that Arctic Chlorella ArM0029B may have a regulatory mechanism of gene expression at earlier steps of the Mg-porphyrin branch that is more similar to higher plants than to the microalga C. reinhardtii. This study provides useful insight into the regulation of porphyrin precursor formation in Chlorella and related microalgae.
Keyword
5-Aminolevulinic acid (ALA)ChlorellaChlorophyllMg-protoporphyrin methyltransferase (MGPMT)Tetrapyrrole
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-017-0424-0
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.