DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seulgi Lee | - |
dc.contributor.author | S J Son | - |
dc.contributor.author | S J Song | - |
dc.contributor.author | Tai Hwan Ha | - |
dc.contributor.author | J S Choi | - |
dc.date.accessioned | 2017-08-29 | - |
dc.date.available | 2017-08-29 | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 2073-4360 | - |
dc.identifier.uri | 10.3390/polym9060224 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/17238 | - |
dc.description.abstract | Because of the complex mechanisms mediating cancer onset, prognosis, and metastatic behavior, different therapeutic approaches targeting these mechanisms have been investigated. Recent advancements in nanocarrier-based drug and gene delivery methods have encouraged scientific groups to investigate various novel therapeutic techniques. In this study, a poly(amidoamine) (PAMAM) polymer-based gene carrier containing the cathepsin B-enzyme sensitive sequence (glycine-phenylalanine-leucine-glycine, GFLG) was evaluated to determine transfection efficiency. Following the GFLG sequence, the surface of PAMAM generation 4 (G4) was conjugated with histidine (H) and arginine (R) for improved endosomal escape and cellular uptake, respectively. The successful synthesis of G4-GLFG-H-R was confirmed by 1H-nuclear magnetic resonance spectroscopy. The polyplex composed of G4-GLFG-H-R and pDNA was simulated by the enzyme cathepsin B and induced endosomal escape of pDNA, which was confirmed by gel electrophoresis. Compared with the G4 control, enzyme-sensitive G4-GLFG-H-R showed higher transfection efficiency and lower cytotoxicity in HeLa cells. These results demonstrated that G4-GLFG-H-R may be a highly potent and efficient carrier for gene therapy applications. | - |
dc.publisher | MDPI | - |
dc.title | Polyamidoamine (PAMAM) dendrimers modified with cathepsin-B cleavable oligopeptides for enhanced gene delivery | - |
dc.title.alternative | Polyamidoamine (PAMAM) dendrimers modified with cathepsin-B cleavable oligopeptides for enhanced gene delivery | - |
dc.type | Article | - |
dc.citation.title | Polymers | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 224 | - |
dc.citation.startPage | 224 | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Seulgi Lee | - |
dc.contributor.affiliatedAuthor | Tai Hwan Ha | - |
dc.contributor.alternativeName | 이슬기 | - |
dc.contributor.alternativeName | 손상재 | - |
dc.contributor.alternativeName | 송수정 | - |
dc.contributor.alternativeName | 하태환 | - |
dc.contributor.alternativeName | 최준식 | - |
dc.identifier.bibliographicCitation | Polymers, vol. 9, no. 6, pp. 224-224 | - |
dc.identifier.doi | 10.3390/polym9060224 | - |
dc.subject.keyword | Arginine | - |
dc.subject.keyword | Cathepsin B | - |
dc.subject.keyword | Gene delivery | - |
dc.subject.keyword | GFLG peptide | - |
dc.subject.keyword | Histidine | - |
dc.subject.keyword | Poly(amidoamine) dendrimer | - |
dc.subject.keyword | Polyplex | - |
dc.subject.local | arginine | - |
dc.subject.local | Arginine | - |
dc.subject.local | cathepsin B | - |
dc.subject.local | Cathepsin B | - |
dc.subject.local | gene delivery | - |
dc.subject.local | Gene delivery | - |
dc.subject.local | GFLG peptide | - |
dc.subject.local | Histidine | - |
dc.subject.local | histidine | - |
dc.subject.local | Poly(amidoamine) dendrimer | - |
dc.subject.local | Polyplex | - |
dc.subject.local | polyplex | - |
dc.description.journalClass | Y | - |
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