Polyamidoamine (PAMAM) dendrimers modified with cathepsin-B cleavable oligopeptides for enhanced gene delivery

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dc.contributor.authorSeulgi Lee-
dc.contributor.authorS J Son-
dc.contributor.authorS J Song-
dc.contributor.authorTai Hwan Ha-
dc.contributor.authorJ S Choi-
dc.date.accessioned2017-08-29-
dc.date.available2017-08-29-
dc.date.issued2017-
dc.identifier.issn2073-4360-
dc.identifier.uri10.3390/polym9060224ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17238-
dc.description.abstractBecause of the complex mechanisms mediating cancer onset, prognosis, and metastatic behavior, different therapeutic approaches targeting these mechanisms have been investigated. Recent advancements in nanocarrier-based drug and gene delivery methods have encouraged scientific groups to investigate various novel therapeutic techniques. In this study, a poly(amidoamine) (PAMAM) polymer-based gene carrier containing the cathepsin B-enzyme sensitive sequence (glycine-phenylalanine-leucine-glycine, GFLG) was evaluated to determine transfection efficiency. Following the GFLG sequence, the surface of PAMAM generation 4 (G4) was conjugated with histidine (H) and arginine (R) for improved endosomal escape and cellular uptake, respectively. The successful synthesis of G4-GLFG-H-R was confirmed by 1H-nuclear magnetic resonance spectroscopy. The polyplex composed of G4-GLFG-H-R and pDNA was simulated by the enzyme cathepsin B and induced endosomal escape of pDNA, which was confirmed by gel electrophoresis. Compared with the G4 control, enzyme-sensitive G4-GLFG-H-R showed higher transfection efficiency and lower cytotoxicity in HeLa cells. These results demonstrated that G4-GLFG-H-R may be a highly potent and efficient carrier for gene therapy applications.-
dc.publisherMDPI-
dc.titlePolyamidoamine (PAMAM) dendrimers modified with cathepsin-B cleavable oligopeptides for enhanced gene delivery-
dc.title.alternativePolyamidoamine (PAMAM) dendrimers modified with cathepsin-B cleavable oligopeptides for enhanced gene delivery-
dc.typeArticle-
dc.citation.titlePolymers-
dc.citation.number6-
dc.citation.endPage224-
dc.citation.startPage224-
dc.citation.volume9-
dc.contributor.affiliatedAuthorSeulgi Lee-
dc.contributor.affiliatedAuthorTai Hwan Ha-
dc.contributor.alternativeName이슬기-
dc.contributor.alternativeName손상재-
dc.contributor.alternativeName송수정-
dc.contributor.alternativeName하태환-
dc.contributor.alternativeName최준식-
dc.identifier.bibliographicCitationPolymers, vol. 9, no. 6, pp. 224-224-
dc.identifier.doi10.3390/polym9060224-
dc.subject.keywordArginine-
dc.subject.keywordCathepsin B-
dc.subject.keywordGene delivery-
dc.subject.keywordGFLG peptide-
dc.subject.keywordHistidine-
dc.subject.keywordPoly(amidoamine) dendrimer-
dc.subject.keywordPolyplex-
dc.subject.localarginine-
dc.subject.localArginine-
dc.subject.localcathepsin B-
dc.subject.localCathepsin B-
dc.subject.localgene delivery-
dc.subject.localGene delivery-
dc.subject.localGFLG peptide-
dc.subject.localHistidine-
dc.subject.localhistidine-
dc.subject.localPoly(amidoamine) dendrimer-
dc.subject.localPolyplex-
dc.subject.localpolyplex-
dc.description.journalClassY-
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Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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