Iloprost supports early development of in vitro-produced porcine embryos through activation of the phosphatidylinositol 3-kinase/AKT signalling pathway

Cited 6 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorPil Soo Jeong-
dc.contributor.authorSeung-Bin Yoon-
dc.contributor.authorSeon A Choi-
dc.contributor.authorBong-Seok Song-
dc.contributor.authorJi-Su Kim-
dc.contributor.authorBo Woong Sim-
dc.contributor.authorYoung-Ho Park-
dc.contributor.authorHae Jun Yang-
dc.contributor.authorSeong-Eun Mun-
dc.contributor.authorYoung-Hyun Kim-
dc.contributor.authorPhilyong Kang-
dc.contributor.authorKang Jin Jeong-
dc.contributor.authorYoungjeon Lee-
dc.contributor.authorYeung Bae Jin-
dc.contributor.authorJae Won Huh-
dc.contributor.authorSang-Rae Lee-
dc.contributor.authorD B Koo-
dc.contributor.authorY I Park-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorKyu Tae Chang-
dc.date.accessioned2017-08-29-
dc.date.available2017-08-29-
dc.date.issued2017-
dc.identifier.issn10313613-
dc.identifier.uri10.1071/RD15391ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17247-
dc.description.abstractDespite evidence of the presence of prostaglandin (PG) I2 in mammalian oviducts, its role in early development of in vitro-produced (IVP) embryos is largely unknown. Thus, in the present study we examined the effects of iloprost, a PGI2 analogue, on the in vitro developmental competence of early porcine embryos and the underlying mechanism(s). To examine the effects of iloprost on the development rate of IVF embryos, iloprost was added to the in vitro culture (IVC) medium and cultured for 6 days. Supplementation of the IVC medium with iloprost significantly improved developmental parameters, such as blastocyst formation rate, the trophectoderm:inner cell mass ratio and cell survival in IVF and parthenogenetically activated (PA) embryos. In addition, post-blastulation development into the expanded blastocyst stage was improved in iloprost-treated groups compared with controls. Interestingly, the phosphatidylinositol 3-kinase (PI3K)/AKT signalling pathway was significantly activated by iloprost supplementation in a concentration-dependent manner (10-1000nM), and the beneficial effects of iloprost on the early development of porcine IVF and PA embryos was completely ablated by treatment with 2.5M wortmannin, a PI3K/AKT signalling inhibitor. Importantly, expression of the PI3K/AKT signalling pathway was significantly reduced in somatic cell nuclear transfer (SCNT) compared with IVF embryos, and iloprost supported the early development of SCNT embryos, as was the case for IVF and PA embryos, suggesting a consistent effect of iloprost on the IVC of IVP porcine embryos. Together, these results indicate that iloprost can be a useful IVC supplement for production of IVP early porcine embryos with high developmental competence-
dc.publisherCSIRO Publishing-
dc.titleIloprost supports early development of in vitro-produced porcine embryos through activation of the phosphatidylinositol 3-kinase/AKT signalling pathway-
dc.title.alternativeIloprost supports early development of in vitro-produced porcine embryos through activation of the phosphatidylinositol 3-kinase/AKT signalling pathway-
dc.typeArticle-
dc.citation.titleReproduction Fertility and Development-
dc.citation.number7-
dc.citation.endPage1318-
dc.citation.startPage1306-
dc.citation.volume29-
dc.contributor.affiliatedAuthorPil Soo Jeong-
dc.contributor.affiliatedAuthorSeung-Bin Yoon-
dc.contributor.affiliatedAuthorSeon A Choi-
dc.contributor.affiliatedAuthorBong-Seok Song-
dc.contributor.affiliatedAuthorJi-Su Kim-
dc.contributor.affiliatedAuthorBo Woong Sim-
dc.contributor.affiliatedAuthorYoung-Ho Park-
dc.contributor.affiliatedAuthorHae Jun Yang-
dc.contributor.affiliatedAuthorSeong-Eun Mun-
dc.contributor.affiliatedAuthorYoung-Hyun Kim-
dc.contributor.affiliatedAuthorPhilyong Kang-
dc.contributor.affiliatedAuthorKang Jin Jeong-
dc.contributor.affiliatedAuthorYoungjeon Lee-
dc.contributor.affiliatedAuthorYeung Bae Jin-
dc.contributor.affiliatedAuthorJae Won Huh-
dc.contributor.affiliatedAuthorSang-Rae Lee-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorKyu Tae Chang-
dc.contributor.alternativeName정필수-
dc.contributor.alternativeName윤승빈-
dc.contributor.alternativeName최선아-
dc.contributor.alternativeName송봉석-
dc.contributor.alternativeName김지수-
dc.contributor.alternativeName심보웅-
dc.contributor.alternativeName박영호-
dc.contributor.alternativeName양해준-
dc.contributor.alternativeName문성은-
dc.contributor.alternativeName김영현-
dc.contributor.alternativeName강필용-
dc.contributor.alternativeName정강진-
dc.contributor.alternativeName이영전-
dc.contributor.alternativeName진영배-
dc.contributor.alternativeName허재원-
dc.contributor.alternativeName이상래-
dc.contributor.alternativeName구덕본-
dc.contributor.alternativeName박영일-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName장규태-
dc.identifier.bibliographicCitationReproduction Fertility and Development, vol. 29, no. 7, pp. 1306-1318-
dc.identifier.doi10.1071/RD15391-
dc.subject.keywordculture medium-
dc.subject.keywordsignal transduction-
dc.subject.localculture medium-
dc.subject.localCulture medium-
dc.subject.localsignal transduction-
dc.subject.localSignal transduction-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.