Enhancement of 2,3-butanediol production from Jerusalem artichoke tuber extract by a recombinant Bacillus sp. strain BRC1 with increased inulinase activity = 바실러스 BRC1을 이용한 돼지감자로부터 2,3-부탄디올 생산

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Title
Enhancement of 2,3-butanediol production from Jerusalem artichoke tuber extract by a recombinant Bacillus sp. strain BRC1 with increased inulinase activity = 바실러스 BRC1을 이용한 돼지감자로부터 2,3-부탄디올 생산
Author(s)
Jang Min Park; Baek Rock Oh; In Yeong Kang; Sun-Yeon HeoJeong-Woo Seo; S M Park; W K Hong; Chul Ho Kim
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 44, no. 7, pp. 1107-1113
Publication Year
2017
Abstract
A Bacillus sp. strain named BRC1 is capable of producing 2,3-butanediol (2,3-BD) using hydrolysates of the Jerusalem artichoke tuber (JAT), a rich source of the fructose polymer inulin. To enhance 2,3-BD production, we undertook an extensive analysis of the Bacillus sp. BRC1 genome, identifying a putative gene (sacC) encoding a fructan hydrolysis enzyme and characterizing the activity of the resulting recombinant protein expressed in and purified from Escherichia coli. Introduction of the sacC gene into Bacillus sp. BRC1 using an expression vector increased enzymatic activity more than twofold. Consistent with this increased enzyme expression, 2,3-BD production from JAT was also increased from 3.98 to 8.10 g L-1. Fed-batch fermentation of the recombinant strain produced a maximal level of 2,3-BD production of 28.6 g L-1, showing a high theoretical yield of 92.3%.
Keyword
2,3-ButanediolBacillus speciesFructan hydrolysisJerusalem artichoke tubersLevanase
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10295-017-1932-1
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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