Reyranella aquatilis sp. nov., an alphaproteobacterium isolated from a eutrophic lake

Abstract

A novel Gram-stain-negative, rod-shaped and non-motile bacterial strain, designated strain Seoho-37T, was isolated from a eutrophic lake in South Korea. Polyphasic studies were performed to investigate the taxonomic position of the new isolate. The isolate grew aerobically with 0-1.0% (w/v) NaCl (optimum 0%), at pH 6.0-10.0 (optimum pH 7.0-9.0) and at temperatures of 15-36°C (optimum 25-30°C) on R2A medium. In the phylogenetic analysis of 16S rRNA gene sequences, strain Seoho-37T formed a clear cluster with the strains of Reyranella graminifolii, Reyranella massiliensis and Reyranella soli with a bootstrap resampling value of 100%. DNA-DNA relatedness between strain Seoho-37T and the type strains of each species in the genus Reyranella was <20%. The genomic DNA G+C content of strain Seoho-37T was 66.5mol%. Ubiquinone-10 (Q-10) and ubiquinone-9 (Q-9) were found as the respiratory quinones. The cellular polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol and phosphatidylmethylethanolamine. The major fatty acid components included C16:0, summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C18:1 2-OH. Based on the above evidence from a polyphasic study, strain Seaho-37T represents a novel species of the genus Reyranella, for which the name Reyranella aquatilis sp. nov. is proposed. The type strain is Seoho-37T (=KCTC 52223T=JCM 31892T).