Silanimonas algicola sp. nov., isolated from laboratory culture of a bloom-forming cyanobacterium, Microcystis

Abstract

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated M23T, was isolated from a laboratory culture of a bloom-forming cyanobacterium, Microcystis, which was isolated from a eutrophic lake in Korea. The strain grew optimally without NaCl and at 25-30 °C on R2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the genus Silanimonas, with the highest similarity to Silanimonas lenta DSM 16282T (98.5 %). DNA-DNA relatedness between strain M23T and the closely related species in the genus Silanimonas was <30 %. Strain M23T contained iso-C15: 0, summed feature 3 (C16: 1ω7c and/or C16: 1ω6c) and iso-C16: 0 as major fatty acids and ubiquinone-8 (Q-8) as the major quinone. Strain M23T contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmethylethanolamine as major polar lipids. The DNA G+C content of strain M23T was 69.6 mol%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain M23T represents a novel species in the genus Silanimonas, for which the name Silanimonas algicola sp. nov. is proposed. The type strain is M23T (=KCTC 52219T=JCM 31889T).