Oryzihumus soli sp. nov., isolated from soil and emended description of the genus Oryzihumus

Abstract

A novel strain designated Aerobe-19T was isolated from a soil sample collected from a lawn located in Seoul National University in Korea. The cells were Gram-stain-positive, aerobic, and coccus-shaped. Colonies were circular with entire edges, convex, opaque and pale yellow. The strain grew at 15-30 °C (optimum, 30 °C), pH 5.0-7.0 (optimum, 6.0) and in the presence of 0-0.5% (w/v) NaCl. Phylogenetically, the strain was found to be closely related to members of the genus Oryzihumus and showed 16S rRNA gene sequence similarities of 96.4 and 96.3% with Oryzihumus leptocrescens NRRL B- 24347T and Oryzihumus terrae KACC 16543T, respectively. The major cellular fatty acids were anteiso-C17: 0, iso-C15: 0, anteiso-C15: 0 and iso-C16: 0. The predominant menaquinone was MK-8 (H4). The polar lipids profile revealed the presence of diphosphatidylglycerol, glycolipids, unknown amino-glycophospholipid, unknown phospholipid and unknown lipids. The peptidoglycan type was A1g. The DNA G+C content of this stain was 73.9 mol%. On the basis of data presented, strain Aerobe-19T is considered to represent a novel species of the genus Oryzihumus, for which the name Oryzihumus soli sp. nov. is proposed. The type strain is Aerobe-19T (=KACC 18485T =KCTC 39705T=NBRC 111450T).