Comprehensive miRNA profiling of skeletal muscle and serum in induced and normal mouse muscle atrophy during aging

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Title
Comprehensive miRNA profiling of skeletal muscle and serum in induced and normal mouse muscle atrophy during aging
Author(s)
H J Jung; Kwang-Pyo Lee; B Milholland; Yeo-Jin Shin; Jae Sook Kang; Ki Sun Kwon; Y Suh
Bibliographic Citation
Journals of Gerontology Series A-Biological Sciences and Medical Sciences, vol. 72, no. 11, pp. 1483-1491
Publication Year
2017
Abstract
Age-associated loss of muscle mass and function is a major cause of morbidity and mortality in the elderly adults. Muscular atrophy can also be induced by disuse associated with long-term bed rest or disease. Although miRNAs regulate muscle growth, regeneration, and aging, their potential role in acute muscle atrophy is poorly understood. Furthermore, alterations in circulating miRNA levels have been shown to occur during aging but their potential as noninvasive biomarkers for muscle atrophy remains largely unexplored. Here, we report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy mouse model, mimicking the acute atrophy following long-term bed rest, as compared to those of young and old mice. Comparative analysis and validation studies have revealed that miR-455-3p was significantly decreased in muscle of both induced-atrophy model and old mice, whereas miR-434-3p was decreased in both serum and muscle of old mice, as compared to young mice. Furthermore, upregulation of miR-455-3p in fully differentiated C2C12 myoblasts induced a hypertrophic phenotype. These results suggest that deregulation of miR-455-3p may play a functional role in muscle atrophy and miR-434-3p could be a candidate serum biomarker of muscle aging.
Keyword
Disuse muscle atrophyMicroRNAMuscle agingSarcopeniaSerum
ISSN
1079-5006
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/gerona/glx025
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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