Identification of the biosynthesis gene cluster for the novel lantibiotic paenilan from Paenibacillus polymyxa E681 and characterization of its product = 패니바실러스 폴리믹사 E681 균의 신규 랜티바이오틱 패니란 생합성 유전자군의 동정 및 패니란의 특성 분석

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dc.contributor.authorJae Eun Park-
dc.contributor.authorHa Rim Kim-
dc.contributor.authorSoo Young Park-
dc.contributor.authorSoo-Keun Choi-
dc.contributor.authorSeung-Hwan Park-
dc.date.accessioned2018-01-11T02:53:04Z-
dc.date.available2018-01-11T02:53:04Z-
dc.date.issued2017-
dc.identifier.issn1364-5072-
dc.identifier.uri10.1111/jam.13580ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17482-
dc.description.abstractAims: To analyse the function of a putative lantibiotic gene cluster of Paenibacillus polymyxa E681 and to characterize its product, paenilan. Methods and Results: Comparative analysis of the lantibiotic gene cluster of E681 revealed that the cluster, consisting of 11 open reading frames, is involved in the biosynthesis of a class I lantibiotic. The pnlA gene encoding the prepeptide PnlA was identified and P. polymyxa strain EPT14 producing only paenilan was constructed by knockout of the other five antibiotic biosynthetic gene clusters of E681. Paenilan was purified from EPT14 culture by solvent partitioning, ODS silica gel chromatography and reversed-phase preparative HPLC. The molecular mass (2510·10 Da) and structure of paenilan analysed by Nanoelectrospray ionization mass spectrometry (MS) and MS/MS showed that paenilan is a novel class I lantibiotic. Paenilan exhibited antimicrobial activity against Gram-positive bacteria such as Bacillus cereus, Micrococcus luteus and Paenibacillus durus. The paenilan gene is well-conserved in different Paenibacillus sp. isolated from globally distant places. Conclusions: The lantibiotic gene cluster of P. polymyxa E681 was analysed and its product, a novel and useful lantibiotic named paenilan that inhibits the growth of some Gram-positive bacteria, was characterized. Significance and Impact of the Study: Paenibacillus species are a good source of new lantibiotics, and the conservation of the paenilan gene among Paenibacillus sp. implies paenilan has an important function(s) for their survival.-
dc.publisherWiley-
dc.titleIdentification of the biosynthesis gene cluster for the novel lantibiotic paenilan from Paenibacillus polymyxa E681 and characterization of its product = 패니바실러스 폴리믹사 E681 균의 신규 랜티바이오틱 패니란 생합성 유전자군의 동정 및 패니란의 특성 분석-
dc.title.alternativeIdentification of the biosynthesis gene cluster for the novel lantibiotic paenilan from Paenibacillus polymyxa E681 and characterization of its product-
dc.typeArticle-
dc.citation.titleJournal of Applied Microbiology-
dc.citation.number5-
dc.citation.endPage1147-
dc.citation.startPage1133-
dc.citation.volume123-
dc.contributor.affiliatedAuthorJae Eun Park-
dc.contributor.affiliatedAuthorHa Rim Kim-
dc.contributor.affiliatedAuthorSoo Young Park-
dc.contributor.affiliatedAuthorSoo-Keun Choi-
dc.contributor.affiliatedAuthorSeung-Hwan Park-
dc.contributor.alternativeName박재은-
dc.contributor.alternativeName김하림-
dc.contributor.alternativeName박수영-
dc.contributor.alternativeName최수근-
dc.contributor.alternativeName박승환-
dc.identifier.bibliographicCitationJournal of Applied Microbiology, vol. 123, no. 5, pp. 1133-1147-
dc.identifier.doi10.1111/jam.13580-
dc.subject.keywordantibiotics-
dc.subject.keywordbacteriocins-
dc.subject.keywordgenes-
dc.subject.keywordidentification-
dc.subject.keywordmolecular genetic-
dc.subject.localantibiotic-
dc.subject.localAntibiotic-
dc.subject.localAntibiotics-
dc.subject.localantibiotics-
dc.subject.localBacteriocin-
dc.subject.localbacteriocins-
dc.subject.localbacteriocin-
dc.subject.localGene-
dc.subject.localgene-
dc.subject.localgenes-
dc.subject.localGenes-
dc.subject.localidentification-
dc.subject.localIdentification-
dc.subject.localmolecular genetics-
dc.subject.localmolecular genetic-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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