A liver-specific gene expression panel predicts the differentiation status of In vitro hepatocyte models

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dc.contributor.authorDae Soo Kim-
dc.contributor.authorJea Woon Ryu-
dc.contributor.authorMi Young Son-
dc.contributor.authorJ H Oh-
dc.contributor.authorKyung-Sook Chung-
dc.contributor.authorSugi Lee-
dc.contributor.authorJeong Ju Lee-
dc.contributor.authorJun Ho Ahn-
dc.contributor.authorJu-Sik Min-
dc.contributor.authorJiwon Ahn-
dc.contributor.authorHyun Mi Kang-
dc.contributor.authorJanghwan Kim-
dc.contributor.authorCho Rok Jung-
dc.contributor.authorNam-Soon Kim-
dc.contributor.authorHyun Soo Cho-
dc.date.accessioned2018-01-11T02:53:07Z-
dc.date.available2018-01-11T02:53:07Z-
dc.date.issued2017-
dc.identifier.issn0270-9139-
dc.identifier.uri10.1002/hep.29324ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17493-
dc.description.abstractAlternative cell sources, such as three-dimensional organoids and induced pluripotent stem cell-derived cells, might provide a potentially effective approach for both drug development applications and clinical transplantation. For example, the development of cell sources for liver cell-based therapy has been increasingly needed, and liver transplantation is performed for the treatment for patients with severe end-stage liver disease. Differentiated liver cells and three-dimensional organoids are expected to provide new cell sources for tissue models and revolutionary clinical therapies. However, conventional experimental methods confirming the expression levels of liver-specific lineage markers cannot provide complete information regarding the differentiation status or degree of similarity between liver and differentiated cell sources. Therefore, in this study, to overcome several issues associated with the assessment of differentiated liver cells and organoids, we developed a liver-specific gene expression panel (LiGEP) algorithm that presents the degree of liver similarity as a “percentage.” We demonstrated that the percentage calculated using the LiGEP algorithm was correlated with the developmental stages of in vivo liver tissues in mice, suggesting that LiGEP can correctly predict developmental stages. Moreover, three-dimensional cultured HepaRG cells and human pluripotent stem cell-derived hepatocyte-like cells showed liver similarity scores of 59.14% and 32%, respectively, although general liver-specific markers were detected. Conclusion: Our study describes a quantitative and predictive model for differentiated samples, particularly liver-specific cells or organoids; and this model can be further expanded to various tissue-specific organoids; our LiGEP can provide useful information and insights regarding the differentiation status of in vitro liver models.-
dc.publisherWiley-
dc.titleA liver-specific gene expression panel predicts the differentiation status of In vitro hepatocyte models-
dc.title.alternativeA liver-specific gene expression panel predicts the differentiation status of In vitro hepatocyte models-
dc.typeArticle-
dc.citation.titleHepatology-
dc.citation.number5-
dc.citation.endPage1674-
dc.citation.startPage1662-
dc.citation.volume66-
dc.contributor.affiliatedAuthorDae Soo Kim-
dc.contributor.affiliatedAuthorJea Woon Ryu-
dc.contributor.affiliatedAuthorMi Young Son-
dc.contributor.affiliatedAuthorKyung-Sook Chung-
dc.contributor.affiliatedAuthorSugi Lee-
dc.contributor.affiliatedAuthorJeong Ju Lee-
dc.contributor.affiliatedAuthorJun Ho Ahn-
dc.contributor.affiliatedAuthorJu-Sik Min-
dc.contributor.affiliatedAuthorJiwon Ahn-
dc.contributor.affiliatedAuthorHyun Mi Kang-
dc.contributor.affiliatedAuthorJanghwan Kim-
dc.contributor.affiliatedAuthorCho Rok Jung-
dc.contributor.affiliatedAuthorNam-Soon Kim-
dc.contributor.affiliatedAuthorHyun Soo Cho-
dc.contributor.alternativeName김대수-
dc.contributor.alternativeName유제운-
dc.contributor.alternativeName손미영-
dc.contributor.alternativeName오정화-
dc.contributor.alternativeName정경숙-
dc.contributor.alternativeName이수기-
dc.contributor.alternativeName이정주-
dc.contributor.alternativeName안준호-
dc.contributor.alternativeName민주식-
dc.contributor.alternativeName안지원-
dc.contributor.alternativeName강현미-
dc.contributor.alternativeName김장환-
dc.contributor.alternativeName정초록-
dc.contributor.alternativeName김남순-
dc.contributor.alternativeName조현수-
dc.identifier.bibliographicCitationHepatology, vol. 66, no. 5, pp. 1662-1674-
dc.identifier.doi10.1002/hep.29324-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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