Roseomonas terricola sp. nov., isolated from agricultural soil

Abstract

A novel strain, designated EM302T, was isolated from a soil sample collected from farmland located in Yesan-gun in the Republic of Korea. The cells were Gram-stain-negative, aerobic, non-motile without flagella and coccobacilli-shaped. Colonies were circular with entire edges, convex, opaque and pink. The strain grew at 15-40 °C (optimum, 35 °C) and pH 6.0-9.0 (optimum, 7.0). The strain grew in R2A medium without the addition of NaCl. Based on 16S rRNA gene sequences analysis, strain EM302T was found to be phylogenetically related to Roseomonas wooponensis WW53T (96.5% 16S rRNA gene sequence similarity), Roseomonas arcticisoli MC 3624T (95.2%), Roseomonas aerilata 5420-30T (94.5%), Roseomonas pecuniae N75T (94.4%) and Roseomonas vinacea CPCC 100056T (94.4%). The major cellular fatty acids were summed feature 8 (C18: 1ω7c and/or C18: 1ω6c) and C16: 0. The predominant respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminolipid. The DNA G+C content of the type strain was 65.7 mol%. On the basis of data presented here, strain EM302T is considered to represent a novel species of the genus Roseomonas, for which the name Roseomonas terricola sp. nov. is proposed. The type strain is EM302T (=KACC 13942T=KCTC 42906T=NBRC 111477T).