An alcohol dehydrogenase gene from Synechocystis sp. confers salt tolerance in transgenic tobacco

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Title
An alcohol dehydrogenase gene from Synechocystis sp. confers salt tolerance in transgenic tobacco
Author(s)
So Young Yi; Seong Sub Ku; H J Sim; S K Kim; Ji Hyun Park; Jae Il Lyu; Eun Jin So; So Yeon Choi; Jonghyun Kim; Myung-Suk Ahn; Suk Weon Kim; Hyunwoo Park; Won Joong Jeong; Y P Lim; Sung Ran Min; Jang Ryol Liu
Bibliographic Citation
Frontiers in Plant Science, vol. 8, pp. 1965-1965
Publication Year
2017
Abstract
Synechocystis salt-responsive gene 1 (sysr1) was engineered for expression in higher plants, and gene construction was stably incorporated into tobacco plants. We investigated the role of Sysr1 [a member of the alcohol dehydrogenase (ADH) superfamily] by examining the salt tolerance of sysr1-overexpressing (sysr1-OX) tobacco plants using quantitative real-time polymerase chain reactions, gas chromatographymass spectrometry, and bioassays. The sysr1-OX plants exhibited considerably increased ADH activity and tolerance to salt stress conditions. Additionally, the expression levels of several stress-responsive genes were upregulated. Moreover, airborne signals from salt-stressed sysr1-OX plants triggered salinity tolerance in neighboring wild-type (WT) plants. Therefore, Sysr1 enhanced the interconversion of aldehydes to alcohols, and this occurrence might affect the quality of green leaf volatiles (GLVs) in sysr1-OX plants. Actually, the Z-3-hexenol level was approximately twofold higher in sysr1-OX plants than in WT plants within 1?2 h of wounding. Furthermore, analyses of WT plants treated with vaporized GLVs indicated that Z-3-hexenol was a stronger inducer of stress-related gene expression and salt tolerance than E-2-hexenal. The results of the study suggested that increased C6 alcohol (Z-3-hexenol) induced the expression of resistance genes, thereby enhancing salt tolerance of transgenic plants. Our results revealed a role for ADH in salinity stress responses, and the results provided a genetic engineering strategy that could improve the salt tolerance of crops.
Keyword
Alcohol dehydrogenaseCyanobacteriaGreen leaf volatiles (GLVs)PrimingSalt toleranceZ-3-hexenol
ISSN
1664-462X
Publisher
Frontiers Media Sa
DOI
http://dx.doi.org/10.3389/fpls.2017.01965
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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