Comparative analysis of human embryonic stem cell derived neural stem cells as an in vitro human model

Cited 12 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJ H Oh-
dc.contributor.authorCho Rok Jung-
dc.contributor.authorMi Ok Lee-
dc.contributor.authorJanghwan Kim-
dc.contributor.authorMi Young Son-
dc.date.accessioned2018-04-19T05:18:26Z-
dc.date.available2018-04-19T05:18:26Z-
dc.date.issued2018-
dc.identifier.issn11073756-
dc.identifier.uri10.3892/ijmm.2017.3298ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17621-
dc.description.abstractAlternative cell models of human neural stem cells (hNScs) have been developed and used for investigations ranging from in vitro experiments to in vivo clinical studies. However, a cell model capable of mimicking the normal' state of hNScs is mandatory in order to extrapolate the results of these studies to humans. In the present study, to select a more suitable hNSc model for developing human-based experimental platforms, two representative hNSc types were compared, namely human embryonic stem cell (hESc)-derived hNScs and ReNcell cX cells, which are well-characterized immortalized hNSc lines. The hNScs, differentiated from hEScs via human neuroectodermal sphere (hNES) formation, recapitulated the molecular and cellular phenotypes of hNScs, including NSc marker expression and terminal neuronal differentiation potential. comparative analyses of the transcriptome profles of the hESC-derived hNESs and ReNcell cX hNScs showed that the differentiated hNESs were analogous to the ReNcell cX cells, as demonstrated by principal component analysis and hierarchical sample clustering. The hNSC-specifc transcriptome was presented, comprising commonly expressed transcripts between hNESs derived from hEScs and ReNcell cX cells. To elucidate the molecular mechanisms associated with the hNSc identity, the hNSC-specifc transcriptome was analyzed using pathway and functional annotation clustering analyses. The results suggested that hESc-derived hNESs, an expandable and accessible cell source, may be used as a relevant hNSc model in a wide range of neurological investigations.-
dc.publisherSpandidos Publ Ltd-
dc.titleComparative analysis of human embryonic stem cell derived neural stem cells as an in vitro human model-
dc.title.alternativeComparative analysis of human embryonic stem cell derived neural stem cells as an in vitro human model-
dc.typeArticle-
dc.citation.titleInternational Journal of Molecular Medicine-
dc.citation.number2-
dc.citation.endPage790-
dc.citation.startPage783-
dc.citation.volume41-
dc.contributor.affiliatedAuthorCho Rok Jung-
dc.contributor.affiliatedAuthorMi Ok Lee-
dc.contributor.affiliatedAuthorJanghwan Kim-
dc.contributor.affiliatedAuthorMi Young Son-
dc.contributor.alternativeName오정화-
dc.contributor.alternativeName정초록-
dc.contributor.alternativeName이미옥-
dc.contributor.alternativeName김장환-
dc.contributor.alternativeName손미영-
dc.identifier.bibliographicCitationInternational Journal of Molecular Medicine, vol. 41, no. 2, pp. 783-790-
dc.identifier.doi10.3892/ijmm.2017.3298-
dc.subject.keywordDifferentiation-
dc.subject.keywordHuman embryonic stem cell-
dc.subject.keywordMicroarray-
dc.subject.keywordNeural sphere-
dc.subject.keywordNeural stem cell-
dc.subject.keywordReNcell-
dc.subject.localDifferentiation-
dc.subject.localdifferentiation-
dc.subject.localHuman embryonic stem cell-
dc.subject.localHuman embryonic stem cells-
dc.subject.localHuman embryonic stem cells (hESCs)-
dc.subject.localhuman embryonic stem cell-
dc.subject.localmicroarry-
dc.subject.localMicroarray-
dc.subject.localNeural sphere-
dc.subject.localNeural stem cell-
dc.subject.localNeural stem cells-
dc.subject.localNeural stem cells (NSCs)-
dc.subject.localneural stem cell-
dc.subject.localReNcell-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.