Long-term stability and integrity of plasmid-based DNA data storage

Cited 19 time in scopus
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dc.contributor.authorH P Nguyen-
dc.contributor.authorJeho Park-
dc.contributor.authorSeon Joo Park-
dc.contributor.authorChang-Soo Lee-
dc.contributor.authorSeungwoo Hwang-
dc.contributor.authorYong Beom Shin-
dc.contributor.authorTai Hwan Ha-
dc.contributor.authorMoonil Kim-
dc.date.accessioned2018-04-19T05:18:27Z-
dc.date.available2018-04-19T05:18:27Z-
dc.date.issued2018-
dc.identifier.issn20734360-
dc.identifier.uri10.3390/polym10010028ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17623-
dc.description.abstractValidation of long-term DNA stability and integrity are essential for the use of DNA in data storage applications. Because of this, we evaluated the plasmid-based DNA data storage in a manner that preserves DNA stability and integrity. A document consisting of 2046 words was encoded with DNA sequences using Perl script, and the encoded DNA sequences were synthesized for information storage. The DNA comprised a total of 22 chemically synthesized DNA fragments with 400 nucleotides each, which were incorporated into a plasmid vector. A long-term DNA stability study demonstrated that 3-year stored plasmid containing text information showed DNA stability at controlled conditions of -20 °C. The plasmid DNA under accelerated aging conditions (AAC) up to 65 °C for 20 days, which corresponds to approximately 20 years of storage at -20 °C, also exhibited no significant differences in DNA stability compared to newly produced plasmid. Also, the 3-year old plasmid stored at -20 °C and the AAC-tested plasmid stored up to 65 °C for 20 days had functional integrity and nucleotide integrity comparable to control sample, thereby allowing for retrieval of the original error-free text data. Finally, the nucleotides were sequenced, and then decoded to retrieve the original data, thereby allowing us to read the text with 100% accuracy, and amplify the DNA with a simple and quick bacterial transformation. To the best of our knowledge, this is the first report on examining the long-term stability and integrity of plasmid-based DNA data storage. Taken together, our results indicate that plasmid DNA data storage can be useful for long-term archival storage to recover the source text in a reproducible and accountable manner-
dc.publisherMDPI-
dc.titleLong-term stability and integrity of plasmid-based DNA data storage-
dc.title.alternativeLong-term stability and integrity of plasmid-based DNA data storage-
dc.typeArticle-
dc.citation.titlePolymers-
dc.citation.number0-
dc.citation.endPage28-
dc.citation.startPage28-
dc.citation.volume10-
dc.contributor.affiliatedAuthorJeho Park-
dc.contributor.affiliatedAuthorSeon Joo Park-
dc.contributor.affiliatedAuthorChang-Soo Lee-
dc.contributor.affiliatedAuthorSeungwoo Hwang-
dc.contributor.affiliatedAuthorYong Beom Shin-
dc.contributor.affiliatedAuthorTai Hwan Ha-
dc.contributor.affiliatedAuthorMoonil Kim-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeName박제호-
dc.contributor.alternativeName박선주-
dc.contributor.alternativeName이창수-
dc.contributor.alternativeName황승우-
dc.contributor.alternativeName신용범-
dc.contributor.alternativeName하태환-
dc.contributor.alternativeName김문일-
dc.identifier.bibliographicCitationPolymers, vol. 10, pp. 28-28-
dc.identifier.doi10.3390/polym10010028-
dc.subject.keywordData storage-
dc.subject.keywordDNA-
dc.subject.keywordIntegrity-
dc.subject.keywordLong-term storage-
dc.subject.keywordPlasmid-
dc.subject.keywordStability-
dc.subject.localData storage-
dc.subject.localDNA-
dc.subject.localIntegrity-
dc.subject.localLong-term storage-
dc.subject.localPlasmid-
dc.subject.localStability-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Korea Bioinformation Center > 1. Journal Articles
Division of Bio Technology Innovation > Core Facility Management Center > 1. Journal Articles
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