Hymenobacter defluvii sp. nov., isolated from wastewater of an acidic water neutralization facility

Abstract

A non-motile, pink-coloured and rod-shaped bacterium, designated strain POA9T, was isolated from a wastewater treatment facility, Republic of Korea. Cells were Gram-reaction-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were C16: 1ω5c, iso-C15: 0, summed feature 3 (C16: 1ω6c and/or C16: 1ω7c) and anteiso-C15: 0. The strain contained MK-7 as the only isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine. The DNA G+C content was 57 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain POA9T forms a distinct evolutionary lineage within the radiation enclosing the members of the genus Hymenobacter, sharing the highest similarity with Hymenobacter actinosclerus CCUG 39621T (95.5% sequence similarity) followed by Hymenobacter seoulensis 16F7GT (95.5 %), Hymenobacter tibetensis XTM003T (95.4 %), Hymenobacter rutilus K2-33028T (95.4 %) and Hymenobacter psychrotolerans Tibet-IIU11T (94.9 %). A number of phenotypic characteristics distinguished strain POA9T from the related members of the genus Hymenobacter. On the basis of the evidence presented in this study, a novel species, Hymenobacter defluvii sp. Nov., is proposed for strain POA9T (=KCTC 52270T=JCM 31658T).