Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway

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Title
Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway
Author(s)
Sun Young Kang; Bo Min Kim; Kyung Taek Heo; Jae-Hyuk JangWon Gon KimYoung-Soo Hong
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 27, no. 12, pp. 2104-2111
Publication Year
2017
Abstract
A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.
Keyword
Artificial biosynthesiscaffeoyl-HSLferuloyl-HSLphenylacetyl homoserine lactone
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.1705.05033
Type
Article
Appears in Collections:
Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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