Sam5, a coumarate 3-hydroxylase from Saccharothrix espanaensis : new insight into the piceatannol production as a resveratrol 3'-hydroxylase = Resveratrol 3'-hydroxylase 효소를 이용한 피세타놀 생산

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Title
Sam5, a coumarate 3-hydroxylase from Saccharothrix espanaensis : new insight into the piceatannol production as a resveratrol 3'-hydroxylase = Resveratrol 3'-hydroxylase 효소를 이용한 피세타놀 생산
Author(s)
Kyung Taek Heo; Sun Young Kang; Jae-Hyuk JangYoung-Soo Hong
Bibliographic Citation
Chemistryselect, vol. 2, no. 28, pp. 8785-8789
Publication Year
2017
Abstract
Piceatannol is an important natural stilbene, which show a broad range of biological activities of medicinal interest. In recent studies, the Sam5 enzyme from Saccharothrix espanaensis was found to catalyze the conversion of p-coumaric acid to caffeic acid as a coumarate 3-hydroxylase (C3H). In this report, we show that purified recombinant Sam5 enzyme exhibited 5.7-fold higher resveratrol ortho-hydroxylation (resveratrol 3’-hydroxylase; R3’H) activity to produce piceatannol compared to the C3H activity. The substrate saturation curve of the Sam5 enzyme for resveratrol is far superior to that for p-coumaric acid. In addition, we constructed a biological platform to produce piceatannol by adding a sam5 gene in a resveratrol artificial biosynthetic pathway that was already made. According to the results, piceatannol production as high as 31.5 ± 6.3 mg/L was achieved
Keyword
biosynthesiscoumarate 3-hydroxylasepiceatannolresveratrol 3’-hydroxylase
ISSN
2365-6549
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/slct.201701969
Type
Article
Appears in Collections:
Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
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