Fabrication of pyrrole-based electrochemical biosensor platform using nanoimprint lithography

Abstract

Many electrochemical biosensors are developed and widely used for the detection of biological responses. However, there is a limitation like increasing the surface area of the working electrode for the improvement of sensitivity. Nanoimprint lithography (NIL) is a simple method that uses stamp and UV or thermally curable resins for fabricating nanopatterns and nanostructures at low cost and with high throughput and high resolution. Therefore, the fabrication of a nanostructure on the working electrode using nanoimprint lithography can improve not only the sensitivity of a biosensor to biological responses by increasing the surface area of the working electrode but also the mass production of nanopatterned electrodes. Here, a conducting polymer-based UV-curable resin is aimed to develop and to use it to fabricate a uniform nanostructure on the working electrode. The 150 nm pillar patterns on a Pt electrode are created using polydimethylsiloxane stamps and KIMM ANT-6HO UV-NIL system and measured electrochemical signals using cyclic voltammetry. Furthermore, glucose oxidase is immobilized on the conducting polymer-imprinted electrode. The developed electrochemical biosensor successfully measures glucose levels across a dynamic range of 0.1 μg mL-1 to 200 mg mL-1 under optimized conditions