Fast and sensitive near-infrared fluorescent probes for ALP detection and 3d printed calcium phosphate scaffold imaging in vivo

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dc.contributor.authorChul Soon Park-
dc.contributor.authorTai Hwan Ha-
dc.contributor.authorMoonil Kim-
dc.contributor.authorN Raja-
dc.contributor.authorH S Yoon-
dc.contributor.authorM J Sung-
dc.contributor.authorOh Seok Kwon-
dc.contributor.authorH Yoon-
dc.contributor.authorChang-Soo Lee-
dc.date.accessioned2018-04-19T05:18:49Z-
dc.date.available2018-04-19T05:18:49Z-
dc.date.issued2018-
dc.identifier.issn09565663-
dc.identifier.uri10.1016/j.bios.2018.01.018ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17692-
dc.description.abstractAlkaline phosphatase (ALP) is a critical biological marker for osteoblast activity during early osteoblast differentiation, but few biologically compatible methods are available for its detection. Here, we describe the discovery of highly sensitive and rapidly responsive novel near-infrared (NIR) fluorescent probes (NIR-Phos-1, NIR-Phos-2) for the fluorescent detection of ALP. ALP cleaves the phosphate group from the NIR skeleton and substantially alters its photophysical properties, therefore generating a large “turn-on” fluorescent signal resulted from the catalytic hydrolysis on fluorogenic moiety. Our assay quantified ALP activity from 0 to 1.0 U mL -1 with a 10 -5-10 -3 U mL -1 limit of detection (LOD), showing a response rate completed within 1.5 min. A potentially powerful approach to probe ALP activity in biological systems demonstrated real-time monitoring using both concentration- and time-dependent variations of endogenous ALP in live cells and animals. Based on high binding affinity to bone tissue of phosphate moiety, bone-like scaffold-based ALP detection in vivo was accessed using NIR probe-labeled three-dimensional (3D) calcium deficient hydroxyapatite (CDHA) scaffolds. They were subcutaneously implanted into mice and monitored ALP signal changes using a confocal imaging system. Our results suggest the possibility of early-stage ALP detection during neo-bone formation inside a bone defect, by in vivo fluorescent evaluation using 3D CDHA scaffolds-
dc.publisherElsevier-
dc.titleFast and sensitive near-infrared fluorescent probes for ALP detection and 3d printed calcium phosphate scaffold imaging in vivo-
dc.title.alternativeFast and sensitive near-infrared fluorescent probes for ALP detection and 3d printed calcium phosphate scaffold imaging in vivo-
dc.typeArticle-
dc.citation.titleBiosensors & Bioelectronics-
dc.citation.number0-
dc.citation.endPage158-
dc.citation.startPage151-
dc.citation.volume105-
dc.contributor.affiliatedAuthorChul Soon Park-
dc.contributor.affiliatedAuthorTai Hwan Ha-
dc.contributor.affiliatedAuthorMoonil Kim-
dc.contributor.affiliatedAuthorOh Seok Kwon-
dc.contributor.affiliatedAuthorChang-Soo Lee-
dc.contributor.alternativeName박철순-
dc.contributor.alternativeName하태환-
dc.contributor.alternativeName김문일-
dc.contributor.alternativeNameRaja-
dc.contributor.alternativeName윤희숙-
dc.contributor.alternativeName성미정-
dc.contributor.alternativeName권오석-
dc.contributor.alternativeName윤현석-
dc.contributor.alternativeName이창수-
dc.identifier.bibliographicCitationBiosensors & Bioelectronics, vol. 105, pp. 151-158-
dc.identifier.doi10.1016/j.bios.2018.01.018-
dc.subject.keywordAlkaline phosphatase-
dc.subject.keywordBioimaging-
dc.subject.keywordFluorescent probe-
dc.subject.keywordNear-infrared-
dc.subject.keywordThree-dimensional scaffold-
dc.subject.localAlkaline phosphatase-
dc.subject.localBioimaging-
dc.subject.localFluorescent probe-
dc.subject.localNear-infrared-
dc.subject.localThree-dimensional scaffold-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > Core Facility Management Center > 1. Journal Articles
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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