High-level recombinant production of squalene using selected Saccharomyces cerevisiae strains

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Title
High-level recombinant production of squalene using selected Saccharomyces cerevisiae strains
Author(s)
Jong Yun Han; Sung-Hwa Seo; Jae Myeong Song; Hong-Weon Lee; Eui Sung Choi
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 45, no. 4, pp. 239-251
Publication Year
2018
Abstract
For recombinant production of squalene, which is a triterpenoid compound with increasing industrial applications, in microorganisms generally recognized as safe, we screened Saccharomyces cerevisiae strains to determine their suitability. A strong strain dependence was observed in squalene productivity among Saccharomyces cerevisiae strains upon overexpression of genes important for isoprenoid biosynthesis. In particular, a high level of squalene production (400 ± 45 mg/L) was obtained in shake flasks with the Y2805 strain overexpressing genes encoding a bacterial farnesyl diphosphate synthase (ispA) and a truncated form of hydroxyl-3-methylglutaryl-CoA reductase (tHMG1). Partial inhibition of squalene epoxidase by terbinafine further increased squalene production by up to 1.9-fold (756 ± 36 mg/L). Furthermore, squalene production of 2011 ± 75 or 1026 ± 37 mg/L was obtained from 5-L fed-batch fermentations in the presence or absence of terbinafine supplementation, respectively. These results suggest that the Y2805 strain has potential as a new alternative source of squalene production
Keyword
Farnesyl diphosphate synthaseHMG-CoA reductaseMetabolic engineeringSaccharomyces cerevisiaeSqualene
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10295-018-2018-4
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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