Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing

Cited 14 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorA A Mokhtarieh-
dc.contributor.authorJieun Lee-
dc.contributor.authorSemi Kim-
dc.contributor.authorMyung Kyu Lee-
dc.date.accessioned2018-04-19T05:19:11Z-
dc.date.available2018-04-19T05:19:11Z-
dc.date.issued2018-
dc.identifier.issn0005-2736-
dc.identifier.uri10.1016/j.bbamem.2018.02.027ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17784-
dc.description.abstractPreviously a scalable and extrusion-free method has been developed for efficient liposomal encapsulation of DNA by twice stepwise mixing of lipids in ethanol and DNA solution using T-shape mixing chamber. In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab′ to siRNA/SMLs enhanced EGFR-specific cell penetration of SMLs and induced siRNA dependent gene silencing. Unexpectedly, the Cy5.5-labeled Fab′ showed almost no in vivo targeting to the xenografted A549 tumors in SCID-NOD mice. However, multiple injection of the unmodified siRNA/SMLs accumulated in the tumors and induced siRNA-dependent in vivo gene silencing. These results demonstrate that the siRNA/SMLs can be used as a siRNA delivery tool for gene therapy-
dc.publisherElsevier-
dc.titlePreparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing-
dc.title.alternativePreparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing-
dc.typeArticle-
dc.citation.titleBiochimica et Biophysica Acta-Biomembranes-
dc.citation.number6-
dc.citation.endPage1325-
dc.citation.startPage1318-
dc.citation.volume1860-
dc.contributor.affiliatedAuthorJieun Lee-
dc.contributor.affiliatedAuthorSemi Kim-
dc.contributor.affiliatedAuthorMyung Kyu Lee-
dc.contributor.alternativeNameMokhtarieh-
dc.contributor.alternativeName이지은-
dc.contributor.alternativeName김세미-
dc.contributor.alternativeName이명규-
dc.identifier.bibliographicCitationBiochimica et Biophysica Acta-Biomembranes, vol. 1860, no. 6, pp. 1318-1325-
dc.identifier.doi10.1016/j.bbamem.2018.02.027-
dc.subject.keywordFab′ preparation and labeling-
dc.subject.keywordGene silencing-
dc.subject.keywordSimple mixing nanoliposome-
dc.subject.keywordTarget specific delivery-
dc.subject.keywordsiRNA encapsulation-
dc.subject.localFab′ preparation and labeling-
dc.subject.localGene silencing-
dc.subject.localgene silencing-
dc.subject.localSimple mixing nanoliposome-
dc.subject.localTarget specific delivery-
dc.subject.localsiRNA encapsulation-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.