DC Field | Value | Language |
---|---|---|
dc.contributor.author | A A Mokhtarieh | - |
dc.contributor.author | Jieun Lee | - |
dc.contributor.author | Semi Kim | - |
dc.contributor.author | Myung Kyu Lee | - |
dc.date.accessioned | 2018-04-19T05:19:11Z | - |
dc.date.available | 2018-04-19T05:19:11Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0005-2736 | - |
dc.identifier.uri | 10.1016/j.bbamem.2018.02.027 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/17784 | - |
dc.description.abstract | Previously a scalable and extrusion-free method has been developed for efficient liposomal encapsulation of DNA by twice stepwise mixing of lipids in ethanol and DNA solution using T-shape mixing chamber. In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab′ to siRNA/SMLs enhanced EGFR-specific cell penetration of SMLs and induced siRNA dependent gene silencing. Unexpectedly, the Cy5.5-labeled Fab′ showed almost no in vivo targeting to the xenografted A549 tumors in SCID-NOD mice. However, multiple injection of the unmodified siRNA/SMLs accumulated in the tumors and induced siRNA-dependent in vivo gene silencing. These results demonstrate that the siRNA/SMLs can be used as a siRNA delivery tool for gene therapy | - |
dc.publisher | Elsevier | - |
dc.title | Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing | - |
dc.title.alternative | Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing | - |
dc.type | Article | - |
dc.citation.title | Biochimica et Biophysica Acta-Biomembranes | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 1325 | - |
dc.citation.startPage | 1318 | - |
dc.citation.volume | 1860 | - |
dc.contributor.affiliatedAuthor | Jieun Lee | - |
dc.contributor.affiliatedAuthor | Semi Kim | - |
dc.contributor.affiliatedAuthor | Myung Kyu Lee | - |
dc.contributor.alternativeName | Mokhtarieh | - |
dc.contributor.alternativeName | 이지은 | - |
dc.contributor.alternativeName | 김세미 | - |
dc.contributor.alternativeName | 이명규 | - |
dc.identifier.bibliographicCitation | Biochimica et Biophysica Acta-Biomembranes, vol. 1860, no. 6, pp. 1318-1325 | - |
dc.identifier.doi | 10.1016/j.bbamem.2018.02.027 | - |
dc.subject.keyword | Fab′ preparation and labeling | - |
dc.subject.keyword | Gene silencing | - |
dc.subject.keyword | Simple mixing nanoliposome | - |
dc.subject.keyword | Target specific delivery | - |
dc.subject.keyword | siRNA encapsulation | - |
dc.subject.local | Fab′ preparation and labeling | - |
dc.subject.local | Gene silencing | - |
dc.subject.local | gene silencing | - |
dc.subject.local | Simple mixing nanoliposome | - |
dc.subject.local | Target specific delivery | - |
dc.subject.local | siRNA encapsulation | - |
dc.description.journalClass | Y | - |
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