Statistical optimization of culture medium for improved production of antimicrobial compound by Streptomyces rimosus AG-P1441

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Title
Statistical optimization of culture medium for improved production of antimicrobial compound by Streptomyces rimosus AG-P1441
Author(s)
Yoonjung JuKwang-Hee Son; C Jin; Byung Soon Hwang; Dong Jin Park; Chang-Jin Kim
Bibliographic Citation
Food Science and Biotechnology, vol. 27, no. 2, pp. 581-590
Publication Year
2018
Abstract
The nutritional requirements for antimicrobial activity of Streptomyces rimosus AG-P1441 were optimized using statistically-based experimental designs at a flask level. Based on a one-factor-at-a-time (OFAT) approach, glucose, corn starch and soybean meal were identified as the carbon and nitrogen sources having a significant effect on antimicrobial productivity. As a result of investigating the effect of glucose concentration, the highest antimicrobial activity was observed at 3% concentration. Response surface methodology (RSM) was then applied to optimize the growth medium components (corn starch, soybean meal, MgCl2 and glutamate). Antimicrobial productivity increased sharply when the medium consisted of 3% glucose, 3.5% corn starch, 2.5% soybean meal, 1.2 mM MgCl2 and 5.9 mM glutamate. The fermentation using optimized culture medium in a 5-L bioreactor allowed a significant increase in antimicrobial activity, evaluated by the paper disc assay, revealed a 29 mm inhibition zone diameter against Phytophthora capsici.
Keyword
BiocontrolMedium optimizationPhytophthora capsiciStreptomyces rimosus AG-P1441
ISSN
1226-7708
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s10068-017-0257-1
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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