Inhibitory effects of compounds and extracts from Ampelopsis brevipedunculata on IL-6-induced STAT3 activation

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Title
Inhibitory effects of compounds and extracts from Ampelopsis brevipedunculata on IL-6-induced STAT3 activation
Author(s)
Hyun Jae JangSeung Jae Lee; Hyung Jin Lim; Kyungsook JungSoyoung Lee; Chan Sun Park; Seung Woong LeeMun Chual Rho
Bibliographic Citation
Biomed Research International, vol. 2018, pp. 3684845-3684845
Publication Year
2018
Abstract
Ampelopsis brevipedunculata (Maxim.) Trautv. (AB), a traditional East Asian medicine, exhibits protective effects against several inflammatory diseases. Our search for an inhibitor of IL-6-induced JAK2/STAT3 activation revealed that AB ethanolic extract (ABE) had a significant inhibitory effect on IL-6-induced STAT3 expression in Hep3B cells. The isolation and purification of an EtOAc-soluble fraction of ABE (ABEA) using reversed-phase high-performance liquid chromatography (RP-HPLC) afforded 17 compounds. The structures of these compounds (1-17) were elucidated based on 1H and 13C nuclear magnetic resonance (NMR) spectroscopy as well as electrospray-ionization mass spectrometry (ESI-MS) data. ABE and ABEA were screened by a luciferase assay using Hep3B cells transfected with the STAT3 reporter gene. ABEA exhibited potent inhibitory effects on IL-6-induced STAT3 expression; moreover, these effects arose from the inhibition of the phosphorylation of the STAT3, JAK2, and ERK proteins in U266 cells. In addition, the compounds isolated from ABEA were measured for their inhibitory effects on IL-6-stimulated STAT3 expression. Of the compounds isolated, betulin showed the greatest inhibitory effects on IL-6-induced STAT3 activation in the luciferase assay (IC50 value: 3.12 μM). Because of its potential for inhibiting STAT3 activation, A. brevipedunculata could be considered a source of compounds of pharmaceutical intere
ISSN
2314-6133
Publisher
Hindawi Ltd
DOI
http://dx.doi.org/10.1155/2018/3684845
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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