Alkaloids from Tetrastigma hemsleyanum and their anti-inflammatory effects on LPS-induced RAW264.7 cells

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Title
Alkaloids from Tetrastigma hemsleyanum and their anti-inflammatory effects on LPS-induced RAW264.7 cells
Author(s)
C Y Wang; Hyun Jae Jang; Y K Han; X D Su; Seung Woong LeeMun Chual Rho; H S Wang; S Y Yang; Y H Kim
Bibliographic Citation
Molecules, vol. 23, no. 6, pp. e1445-e1445
Publication Year
2018
Abstract
Alkaloids 1-10 were isolated from the aerial parts of Tetrastigma hemsleyanum (APTH) and obtained from species of the genus Tetrastigma for the first time. The chemical structures of the isolated compounds were identified by NMR, UV, and MS analyses. Their anti-inflammatory activities were investigated by measuring nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Among all the isolates, compounds 6, 7 and 10 showed potent inhibitory activity against LPS-stimulated NO production in RAW264.7 cells (IC50: 31.9, 25.2 and 6.3 μM, respectively). Furthermore, APTH and S-(-)-trolline (10) inhibited induction of inflammatory cytokines or mediators such as interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) via suppression of nuclear factor κB (NF- κB) translocation into the nucleus. In addition, 10 suppressed extracellular signal-regulated protein kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) phosphorylation in a dose-dependent manner. These results conclusively demonstrated that compound 10 displays anti-inflammatory activity via suppression of NF- κB activation and the ERK-MAPK signaling pathway in LPS-stimulated RAW264.7 cells
Keyword
AlkaloidAnti-inflammatory activityNO productionTetrastigma hemsleyanum
ISSN
1420-3049
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/molecules23061445
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
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