The use of beta-tubulin gene for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei (EHP) and in the development of a nested PCR as its diagnostic tool

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dc.contributor.authorJ E Han-
dc.contributor.authorK F J Tang-
dc.contributor.authorJi Hyung Kim-
dc.date.accessioned2018-07-19T16:30:47Z-
dc.date.available2018-07-19T16:30:47Z-
dc.date.issued2018-
dc.identifier.issn0044-8486-
dc.identifier.uri10.1016/j.aquaculture.2018.06.059ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17962-
dc.description.abstractTubulins are major components of microtubules, structures that are involved in many cellular processes. The gene β-tubulin, which encodes a β-tubulin protein, is conserved, and yet with sufficient variability to be useful for phylogenetic studies and for use in molecular diagnostic protocols. We sequenced and analyzed a fragment (870-bp) of β-tubulin gene of Enterocytozoon hepatopenaei (EHP), a microsporidian parasite infecting penaeid shrimp and associated with growth retardation. The sequence was then used to conduct a phylogenetic study to determine the position of EHP within the Microsporidia and to develop primers for an EHP nested PCR method. Blast analysis revealed a 75% nucleotide identity of EHP β-tubulin to that of E. bieneusi. Similar to other studies based on the small subunit rRNA sequence, the phylogenetic analysis using β-tubulin sequences clustered EHP with E. bieneusi and two other crustacean microsporidia, Agmasoma penaeii and Hepatospora eriocheir. As for the diagnostic use, a nested PCR method was developed that successfully detected EHP in infected Penaeus vannamei from Indonesia, Thailand, Vietnam, and India and in some cases of Artemia biomass. There were no cross-reactions to the shrimp and other crustacean genomes. This nested PCR is 100-fold more sensitive than the one-step PCR and thus suitable for detecting a low level of EHP infection-
dc.publisherElsevier-
dc.titleThe use of beta-tubulin gene for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei (EHP) and in the development of a nested PCR as its diagnostic tool-
dc.title.alternativeThe use of beta-tubulin gene for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei (EHP) and in the development of a nested PCR as its diagnostic tool-
dc.typeArticle-
dc.citation.titleAquaculture-
dc.citation.number0-
dc.citation.endPage902-
dc.citation.startPage899-
dc.citation.volume495-
dc.contributor.affiliatedAuthorJi Hyung Kim-
dc.contributor.alternativeName한재은-
dc.contributor.alternativeNameTang-
dc.contributor.alternativeName김지형-
dc.identifier.bibliographicCitationAquaculture, vol. 495, pp. 899-902-
dc.identifier.doi10.1016/j.aquaculture.2018.06.059-
dc.subject.keywordEnterocytozoon hepatopenaei-
dc.subject.keywordNested PCR-
dc.subject.keywordShrimp disease-
dc.subject.keywordβ-Tubulin-
dc.subject.localEnterocytozoon hepatopenaei-
dc.subject.localNested PCR-
dc.subject.localShrimp disease-
dc.subject.localβ-Tubulin-
dc.description.journalClassY-
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Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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