DC Field | Value | Language |
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dc.contributor.author | J E Han | - |
dc.contributor.author | K F J Tang | - |
dc.contributor.author | Ji Hyung Kim | - |
dc.date.accessioned | 2018-07-19T16:30:47Z | - |
dc.date.available | 2018-07-19T16:30:47Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0044-8486 | - |
dc.identifier.uri | 10.1016/j.aquaculture.2018.06.059 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/17962 | - |
dc.description.abstract | Tubulins are major components of microtubules, structures that are involved in many cellular processes. The gene β-tubulin, which encodes a β-tubulin protein, is conserved, and yet with sufficient variability to be useful for phylogenetic studies and for use in molecular diagnostic protocols. We sequenced and analyzed a fragment (870-bp) of β-tubulin gene of Enterocytozoon hepatopenaei (EHP), a microsporidian parasite infecting penaeid shrimp and associated with growth retardation. The sequence was then used to conduct a phylogenetic study to determine the position of EHP within the Microsporidia and to develop primers for an EHP nested PCR method. Blast analysis revealed a 75% nucleotide identity of EHP β-tubulin to that of E. bieneusi. Similar to other studies based on the small subunit rRNA sequence, the phylogenetic analysis using β-tubulin sequences clustered EHP with E. bieneusi and two other crustacean microsporidia, Agmasoma penaeii and Hepatospora eriocheir. As for the diagnostic use, a nested PCR method was developed that successfully detected EHP in infected Penaeus vannamei from Indonesia, Thailand, Vietnam, and India and in some cases of Artemia biomass. There were no cross-reactions to the shrimp and other crustacean genomes. This nested PCR is 100-fold more sensitive than the one-step PCR and thus suitable for detecting a low level of EHP infection | - |
dc.publisher | Elsevier | - |
dc.title | The use of beta-tubulin gene for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei (EHP) and in the development of a nested PCR as its diagnostic tool | - |
dc.title.alternative | The use of beta-tubulin gene for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei (EHP) and in the development of a nested PCR as its diagnostic tool | - |
dc.type | Article | - |
dc.citation.title | Aquaculture | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 902 | - |
dc.citation.startPage | 899 | - |
dc.citation.volume | 495 | - |
dc.contributor.affiliatedAuthor | Ji Hyung Kim | - |
dc.contributor.alternativeName | 한재은 | - |
dc.contributor.alternativeName | Tang | - |
dc.contributor.alternativeName | 김지형 | - |
dc.identifier.bibliographicCitation | Aquaculture, vol. 495, pp. 899-902 | - |
dc.identifier.doi | 10.1016/j.aquaculture.2018.06.059 | - |
dc.subject.keyword | Enterocytozoon hepatopenaei | - |
dc.subject.keyword | Nested PCR | - |
dc.subject.keyword | Shrimp disease | - |
dc.subject.keyword | β-Tubulin | - |
dc.subject.local | Enterocytozoon hepatopenaei | - |
dc.subject.local | Nested PCR | - |
dc.subject.local | Shrimp disease | - |
dc.subject.local | β-Tubulin | - |
dc.description.journalClass | Y | - |
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