Development of a kit for diagnosing AtCYP78A7 protein in abiotic-tolerant transgenic rice overexpressing AtCYP78A7

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorK H Nam-
dc.contributor.authorJung Ho Park-
dc.contributor.authorIn Soon Pack-
dc.contributor.authorH B Kim-
dc.contributor.authorChang-Gi Kim-
dc.date.accessioned2018-10-24T16:30:10Z-
dc.date.available2018-10-24T16:30:10Z-
dc.date.issued2018-
dc.identifier.issnI0000128-
dc.identifier.uri10.5352/JLS.2018.28.7.835ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/17994-
dc.description.abstractQuantitative determination of the protein expression levels is one of the most important parts in assessment of the safety of foods derived from genetically modified (GM) crops. Overexpression of AtCYP78A7, a gene encoding cytochrome P450 protein, has been reported to improve tolerance to abiotic stress, such as drought and salt stress, in transgenic rice (Oryza sativa L.). In the present study, an enzyme-linked immunosorbent assay (ELISA) kit for diagnosing AtCYP78A7 protein including AtCYP78A7-specific monoclonal antibody was developed. GST-AtCYP78A7 recombinant protein was induced and purified by affinity column. Four monoclonal antibodies (mAb 6A7, mAb 4C2, mAb 11H6, and mAb 7E8) against recombinant protein were also produced and biotinylated with avidin-HRP. After pairing test using GST-AtCYP78A7 protein and lysate of rice samples, mAb 4C2 and mAb 7E8 were selected as a capture antibody and a detecting antibody, respectively, for ELISA kit. Product test using rice samples indicated that percentages of detected protein in total protein were greater than 0.1% in AtCYP78A7-overexpressing transgenic rice (Line 10B-5 and 18A-4), whereas those in negative control non-transgenic rice (Ilpum and Hwayoung) were less than 0.1%. The ELISA kit developed in this study can be useful for the rapid detection and safety assessment of transgenic rice overexpressing AtCYP78A7.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleDevelopment of a kit for diagnosing AtCYP78A7 protein in abiotic-tolerant transgenic rice overexpressing AtCYP78A7-
dc.title.alternativeDevelopment of a kit for diagnosing AtCYP78A7 protein in abiotic-tolerant transgenic rice overexpressing AtCYP78A7-
dc.typeArticle-
dc.citation.titleJournal of Life Science-
dc.citation.number7-
dc.citation.endPage840-
dc.citation.startPage835-
dc.citation.volume28-
dc.contributor.affiliatedAuthorJung Ho Park-
dc.contributor.affiliatedAuthorIn Soon Pack-
dc.contributor.affiliatedAuthorChang-Gi Kim-
dc.contributor.alternativeName남경희-
dc.contributor.alternativeName박정호-
dc.contributor.alternativeName백인순-
dc.contributor.alternativeName김호방-
dc.contributor.alternativeName김창기-
dc.identifier.bibliographicCitationJournal of Life Science, vol. 28, no. 7, pp. 835-840-
dc.identifier.doi10.5352/JLS.2018.28.7.835-
dc.subject.keywordELISA-
dc.subject.keywordgenetically modifided crop-
dc.subject.keywordmonoclonal antibody-
dc.subject.keywordsafety assessment-
dc.subject.localELISA-
dc.subject.localgenetically modifided crop-
dc.subject.localGenetically modified (GM) crop-
dc.subject.localmonoclonal antibody-
dc.subject.localMonoclonal antibody-
dc.subject.localsafety assessment-
dc.description.journalClassN-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.