DSG2 is a functional cell surface marker for identification and isolation of human pluripotent stem cells

Cited 8 time in scopus
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Title
DSG2 is a functional cell surface marker for identification and isolation of human pluripotent stem cells
Author(s)
Jongjin Park; Y Son; Na Geum Lee; Kungmin Lee; Dong Gwang Lee; Jinhoi Song; Jaemin Lee; Seokho Kim; Min Ji Jo; Ju Hong Jang; Jangwook LeeJong Gil ParkYeon-Gu Kim; Jang Seong Kim; Jungwoon LeeYee Sook ChoYoung-Jun ParkBaek Soo HanKwang-Hee Bae; S Han; B Kang; S Haam; Sang Hyun LeeSang Chul LeeJeong Ki Min
Bibliographic Citation
Stem Cell Reports, vol. 11, pp. 115-127
Publication Year
2018
Abstract
Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.
Keyword
EMTcell surface markerdesmoglein-2monoclonal antibodypluripotent stem cells
ISSN
2213-6711
Publisher
Elsevier-Cell Press
DOI
http://dx.doi.org/10.1016/j.stemcr.2018.05.009
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Research on National Challenges > Biodefense Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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