The effects of MeoH extract of Hopea chinensis (Merr.) Hand.-Mazz. on the metabolism of amyloid precursor protein in neuroblastoma cells = Hopea chinensis (Merr.) Hand.-Mazz. 메탄올 추출물이 신경세포에서 아밀로이드 전구 단백질 대사에 미치는 영향

Abstract

Many plant derived phytochemicals have been considered as the main therapeutic strategy against Alzheimer’s disease (AD). AD is a progressive neurodegenerative disorder, and the most predominant cause of dementia in the elderly. Cholinergic deficit, senile plaque/β-amyloid (Aβ) peptide deposition and oxidative stress have been identified as three main pathogenic pathways which contribute to the progression of AD. We screened many different plant species for their effective use in both modern and traditional system of medicines. In this study, we tested that MeOH extract of the stem bark of Hopea chinensis (Merr.) Hand.-Mazz. (HCM) affects on the processing of Amyloid precursor portein (APP) from the APPswe over-expressing Neuro2a cell line. We showed that HCM reduced the secretion level of Aβ42 and Aβ40 in a dose dependent manner. We found that HCM increased over 1.5 folds of the secretion level of sAPPα, a metabolite of α-secretase. Furthermore, we found that HCM inhibited acetylcholinesterase activity in vitro. We suggest that the stem bark of Hopea chinensis may be a useful source to develop a therapeutics for AD.