Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution

Cited 21 time in scopus
Metadata Downloads
Title
Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution
Author(s)
K A Baritugo; H T Kim; Y David; T U Khang; S M Hyun; K H Kang; J H Yu; Jong Hyun ChoiJae Jun Song; J C Joo; S J Park
Bibliographic Citation
Microbial Cell Factories, vol. 17, pp. 129-129
Publication Year
2018
Abstract
Background: Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gamma-aminobutyrate (GABA), a versatile bulk chemical used in food and pharmaceutical industry, is also used as a key monomer for nylon 4. GABA can be biologically produced by decarboxylation of glutamate. Results: In this study, we examined high glutamate-producing Corynebacterium glutamicum strains as hosts for enhanced production of GABA from glucose and xylose as carbon sources. An Escherichia coli gadB mutant with a broad pH range of activity and E. coli xylAB genes were expressed under the control of a synthetic H36 promoter. When empty fruit bunch (EFB) solution was used as carbon source (45g/L glucose and 5g/L xylose), 12.54±0.07g/L GABA was produced by recombinant C. glutamicum H36GD1852 expressing E. coli gadB mutantgene and xylAB genes. Batch fermentation of the same strain resulted in the production of 35.47g/L of GABA when EFB solution was added to support 90g/L glucose and 10g/L xylose. Conclusions: This is the first report of GABA production by recombinant C. glutamicum strains from co-utilization of glucose and xylose from EFB solution. Recombinant C. glutamicum strains developed in this study should be useful for an efficient and sustainable production of GABA from lignocellulosic biomasses.
Keyword
Co-utilizationCorynebacterium glutamicumEmpty fruit bunchGamma-aminobutyrateXylose
ISSN
1475-2859
Publisher
Springer-BMC
DOI
http://dx.doi.org/10.1186/s12934-018-0977-9
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.