Low-pH production of D-lactic acid using newly isolated acid tolerant yeast Pichia kudriavzevii NG7

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Title
Low-pH production of D-lactic acid using newly isolated acid tolerant yeast Pichia kudriavzevii NG7
Author(s)
Hyun Joo Park; Jung Hoon Bae; Hyeok Jin Ko; Sun Hee Lee; Bong Hyun Sung; J I Han; Jung Hoon Sohn
Bibliographic Citation
Biotechnology and Bioengineering, vol. 115, no. 9, pp. 2232-2242
Publication Year
2018
Abstract
Lactic acid is a platform chemical for the sustainable production of various materials. To develop a robust yeast platform for low-pH production of d-lactic acid (LA), an acid-tolerant yeast strain was isolated from grape skins and named Pichia kudriavzevii NG7 by ribosomal RNA sequencing. This strain could grow at pH 2.0 and 50°C. For the commercial application of P. kudriavzevii NG7 as a lactic acid producer, the ethanol fermentation pathway was redirected to lactic acid by replacing the pyruvate decarboxylase 1 gene (PDC1) with the d-lactate dehydrogenase gene (d-LDH) derived from Lactobacillus plantarum. To enhance lactic acid tolerance, this engineered strain was adapted to high lactic acid concentrations, and a new transcriptional regulator, PAR1, responsible for acid tolerance, was identified by whole-genome resequencing. The final engineered strain produced 135g/L and 154g/L of d-LA with productivity over 3.66g/L/hr at pH 3.6 and 4.16g/L/hr at pH 4.7, respectively.
Keyword
Pichia kudriavzeviiacid tolerancelactic acid (LA)
ISSN
0006-3592
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/bit.26745
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
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