Ulleungdin, a lasso peptide with cancer cell migration inhibitory activity discovered by the genome mining approach = 게놈 마이닝에 의해 발굴된 암전이 억제제, 울릉딘

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Title
Ulleungdin, a lasso peptide with cancer cell migration inhibitory activity discovered by the genome mining approach = 게놈 마이닝에 의해 발굴된 암전이 억제제, 울릉딘
Author(s)
Sangkeun Son; Mina Jang; Byungsan Lee; Young-Soo HongSung-Kyun KoJae-Hyuk JangJong Seog Ahn
Bibliographic Citation
Journal of Natural Products, vol. 81, no. 10, pp. 2205-2211
Publication Year
2018
Abstract
The advances of genomic sequence analyses and genome mining tools have enabled the exploration of untapped microbial natural products. Through genome mining studies to discover cryptic natural products, we found biosynthetic genes encoding a new lasso peptide in the genome sequence of a soil bacterium, Streptomyces sp. KCB13F003 isolated from Ulleung Island (a small volcanic island), Korea. The production and purification of the encoded peptide, named ulleungdin, were achieved by optimizing the culture conditions followed by LC-MS-targeted isolation. Structure elucidation was performed by NMR spectroscopic and MS spectrometric analyses and chemical means (Marfey's and GITC derivatizations), proving ulleungdin to be a new 15-mer class II lasso peptide with a threaded structure. Biological evaluation with the cell invasion assay and time-lapse cell tracking analysis revealed that ulleungdin has significant inhibitory activities against cancer cell invasion and migration.
ISSN
0163-3864
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/acs.jnatprod.8b00449
Type
Article
Appears in Collections:
Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
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