Construction of a Vitreoscilla hemoglobin promoter-based tunable expression system for Corynebacterium glutamicum = 비트레오실라 헤모글로빈 프로모터 이용 코리네박테리움 글루타미컴 단백질 발현시스템 구축

Cited 7 time in scopus
Metadata Downloads
Title
Construction of a Vitreoscilla hemoglobin promoter-based tunable expression system for Corynebacterium glutamicum = 비트레오실라 헤모글로빈 프로모터 이용 코리네박테리움 글루타미컴 단백질 발현시스템 구축
Author(s)
K A Baritugo; H T Kim; M N Rhie; S Y Jo; T U Khang; K H Kang; S K Song; Binna LeeJae Jun SongJong Hyun ChoiDae-Hee Lee; J C Joo; S J Park
Bibliographic Citation
Catalysts, vol. 8, pp. 561-561
Publication Year
2018
Abstract
Corynebacterium glutamicum is an industrial strain used for the production of valuable chemicals such as L-lysine and L-glutamate. Although C. glutamicum has various industrial applications, a limited number of tunable systems are available to engineer it for efficient production of platform chemicals. Therefore, in this study, we developed a novel tunable promoter system based on repeats of the Vitreoscilla hemoglobin promoter (Pvgb). Tunable expression of green fluorescent protein (GFP) was investigated under one, four, and eight repeats of Pvgb (Pvgb, Pvgb4, and Pvgb8). The intensity of fluorescence in recombinant C. glutamicum strains increased as the number of Pvgb increased from single to eight (Pvgb8) repeats. Furthermore, we demonstrated the application of the new Pvgb promoter-based vector system as a platform for metabolic engineering of C. glutamicum by investigating 5-aminovaleric acid (5-AVA) and gamma-aminobutyric acid (GABA) production in several C. glutamicum strains. The profile of 5-AVA and GABA production by the recombinant strains were evaluated to investigate the tunable expression of key enzymes such as DavBA and GadBmut. We observed that 5-AVA and GABA production by the recombinant strains increased as the number of Pvgb used for the expression of key proteins increased. The recombinant C. glutamicum strain expressing DavBA could produce higher amounts of 5-AVA under the control of Pvgb8 (3.69 ± 0.07 g/L) than the one under the control of Pvgb (3.43 ± 0.10 g/L). The average gamma-aminobutyric acid production also increased in all the tested strains as the number of Pvgb used for GadBmut expression increased from single (4.81?5.31 g/L) to eight repeats (4.94?5.58 g/L).
Keyword
Corynebacterium glutamicumExpression vectorsPvgbSynthetic biologyTunable expression systemVgbVitreoscilla
ISSN
2073-4344
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/catal8110561
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.