TXNIP regulates AKT-mediated cellular senescence by direct interaction under glucose-mediated metabolic stress

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Title
TXNIP regulates AKT-mediated cellular senescence by direct interaction under glucose-mediated metabolic stress
Author(s)
Hangsak Huy; Hae-Young Song; Mi Jeong Kim; Won Sam Kim; Dong Oh Kim; Jae-Eun Byun; Jungwoon LeeYoung-Jun ParkTae-Don KimSuk Ran Yoon; E J Choi; Chul-Ho LeeJi-Yoon NohHaiyoung Jung; In Pyo Choi
Bibliographic Citation
Aging Cell, vol. 17, no. 6, pp. e12836-e12836
Publication Year
2018
Abstract
Aging is associated with an inevitable and universal loss of cell homeostasis and restricts an organism's lifespan by an increased susceptibility to diseases and tissue degeneration. The glucose uptake associated with producing energy for cell survival is one of the major causes of ROS production under physiological conditions. However, the overall mechanisms by which glucose uptake results in cellular senescence remain mysterious. In this study, we found that TXNIP deficiency accelerated the senescent phenotypes of MEF cells under high glucose condition. TXNIP-/- MEF cells showed greater induced glucose uptake and ROS levels than wild-type cells, and N-acetylcysteine (NAC) treatment rescued the cellular senescence of TXNIP-/- MEF cells. Interestingly, TXNIP-/- MEF cells showed continuous activation of AKT during long-term subculture, and AKT signaling inhibition completely blocked the cellular senescence of TXNIP-/- MEF cells. In addition, we found that TXNIP interacted with AKT via the PH domain of AKT, and their interaction was increased by high glucose or H2 O2 treatment. The inhibition of AKT activity by TXNIP was confirmed using western blotting and an in vitro kinase assay. TXNIP deficiency in type 1 diabetes mice (Akita) efficiently decreased the blood glucose levels and finally increased mouse survival. However, in normal mice, TXNIP deficiency induced metabolic aging of mice and cellular senescence of kidney cells by inducing AKT activity and aging-associated gene expression. Altogether, these results suggest that TXNIP regulates cellular senescence by inhibiting AKT pathways via a direct interaction under conditions of glucose-derived metabolic stress.
Keyword
AKTTXNIPagingglucosereactive oxygen species
ISSN
1474-9718
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/acel.12836
Type
Article
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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